A Window on the Study of Aversive Instrumental Learning: Strains, Performance, Neuroendocrine, and Immunologic Systems

Autor(es)

de Oliveira, Caroline C. ; Gouveia, Flavia V. ; de Castro, Marina C. ; Kuroki, Mayra A. ; dos Santos, Lennon C. T. ; Fonoff, Erich T. ; Teixeira, Manoel J. ; Otoch, Jose P. ; Martinez, Raquel C. R.

• Tipo de documento: Artigo Científico
• Fonte: FRONTIERS IN BEHAVIORAL NEUROSCIENCE; v. 10, AUG 24 2016.
• Citações Web of Science: 0

Resumo

The avoidance response is present in pathological anxiety and interferes with normal daily functions. The aim of this article is to shed light on performance markers of active avoidance (AA) using two different rat strains, Sprague-Dawley (SD) and Wistar. Specifically, good and poor performers were evaluated regarding anxiety traits exhibited in the elevated plus maze (EPM) and corticosterone levels and motor activity in the open field test. In addition, the plasma levels of Interleukin-6 (IL-6), Interleukin-1 Beta (IL-1 beta), Nerve Growth Factor Beta (NGF-beta), Tumor Necrosis Factor-Alpha (TNF-alpha) and cytokine-induced neutrophil chemoattractant 1 (CINC-1) were compared in the good and poor performers to better understand the role of the immunologic system in aversive learning. Behavioral criteria were employed to identify subpopulations of SD and Wistar rats based on their behavioral scores during a two-way AA test. The animals were tested for anxiety-like behavior in the EPM and motor activity in the open-field test. Plasma corticosterone levels were measured at the end of the avoidance test. Cytokine levels of IL-6, IL-1 beta, NGF-beta, TNF-alpha, and CINC-1 were measured in the plasma of the Wistar rats. Sixty-six percent of the Wistar rats and 35% of the SD rats exhibited a poor performance. This feature was associated with a decrease in anxiety-like behavior in the EPM. The poor and good performers exhibited lower levels of corticosterone compared with the control animals, which suggests that training alters corticosterone levels, thereby leading to hypocortisolism, independent of the performance. The CINC-1 levels were increased in the poor performers, which reinforces the role of immunologic system activation in learning deficits. Our study provides a better understanding of the complex interactions that underlie neuroimmune consequences and their implications for performance. (AU)

• Processo FAPESP: 11/08575-7 - Papel da modulação dopaminérgica no núcleo lateral da amígdala sobre a resposta condicionada de esquiva
• Beneficiário: Raquel Chacon Ruiz Martinez
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 14/12999-5 - Avaliação do fator linhagem nos níveis de ansiedade em animais com desempenho bom e ruim no teste da aprendizagem aversiva: Wistar versus Sprague Dawley
• Beneficiário: Lennon Tosati Cardoso Dos Santos
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 13/20602-5 - Análise prospectiva dos resultados funcionais da amigdalotomia bilateral assistencial em pacientes com agressividade refratária
• Beneficiário: Flavia Venetucci Gouveia
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 13/03039-5 - Papel dos níveis plasmáticos de corticosterona em animais com desempenho bom e ruim no teste da aprendizagem aversiva
• Beneficiário: Caroline Cruz de Oliveira
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 13/03040-3 - Investigação dos níveis de ansiedade com o desempenho no teste da aprendizagem aversiva
• Beneficiário: Marina Correia de Castro
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica