Immune Response of Green Sea Turtles with and without Fibropapillomatosis: Evaluating Oxidative Burst and Phagocytosis via Flow Cytometry

Autor(es)

Rossi, Silmara ; de Queiroz Hazarbassanov, Nicolle G. T. ; Sanchez-Sarmiento, Angelica Maria ; Setim Prioste, Fabiola Eloisa ; Matushima, Eliana Reiko

• Tipo de documento: Artigo Científico
• Fonte: CHELONIAN CONSERVATION AND BIOLOGY; v. 15, n. 2, p. 273-278, DEC 2016.
• Citações Web of Science: 0

Resumo

Fibropapillomatosis (FP) has a complex etiology, involving genetic and environmental factors, and is considered a threat to green sea turtles (Chelonia mydas). The goals of this study were to evaluate phagocytosis and oxidative burst in blood samples of green sea turtles with and without FP. We analyzed samples from 38 specimens (27 with FP) captured at a feeding area in Brazil. No differences were observed between specimens with and without FP regarding leukocyte activity; nevertheless, the analyses revealed there were significant differences among leukocyte populations of animals with FP, lymphocytes and monocytes had higher phagocytic activity than did granulocytes, and lymphocytes had lower oxidative burst activity than did granulocytes and monocytes. This study described an efficient method to assess leukocyte activity through flow cytometry and revealed important characteristics of white blood cells from green sea turtles with FP. (AU)

• Processo FAPESP: 11/04565-7 - Presença de contaminantes ambientais em tecidos de tartaruga-verde (Chelonia mydas) provenientes da Costa brasileira: estudo da prevalência em animais acometidos pela fibropapilomatose
• Beneficiário: Angelica Maria Sanchez Sarmiento
• Linha de fomento: Bolsas no Brasil - Mestrado
• Processo FAPESP: 10/01781-8 - Avaliação da função celular de leucócitos por citometria de fluxo e a influência de bifenilos policlorados no desenvolvimento da fibropapilomatose em Chelonia mydas (Linnaeus, 1758) (Testudines, Cheloniidae).
• Beneficiário: Silmara Rossi
• Linha de fomento: Bolsas no Brasil - Doutorado
• Processo FAPESP: 12/14319-6 - Ecologia e etiologia da fibropapilomatose em populações de Chelonia mydas (Testudines, Cheloniidae): estrutura das populações e papel de bifenilos policlorados e metais potencialmente tóxicos no desenvolvimento da doença
• Beneficiário: Eliana Reiko Matushima
• Linha de fomento: Auxílio à Pesquisa - Regular