Alterations of Intestinal Microbiome by Antibiotic Therapy in Hospitalized Children

Autor(es)

Fernandes, Miriam R. ; Ignacio, Aline ; Rodrigues, Viviane A. A. ; Groppo, Franciso C. ; Cardoso, Ary L. ; Avila-Campos, Mario J. ; Nakano, Viviane

• Tipo de documento: Artigo Científico
• Fonte: MICROBIAL DRUG RESISTANCE; v. 23, n. 1, p. 56+, JAN 2017.
• Citações Web of Science: 2

Resumo

The administration of antimicrobial agents leads to an ecological imbalance of the host-microorganisms relationship, and it causes a rapid and significant reduction in the microbial diversity. The aim of the current study was to evaluate the impact of antibiotic therapy on intestinal microbiota of children between 3 and 12 years of age. The fecal samples were collected from hospitalized children (n = 31) and from healthy untreated children (n = 30). The presence of bacteria and their quantities were assessed by culture-based methods and quantitative polymerase chain reaction (qPCR). By culture method, in the children receiving antibiotics, a low recovery of Bifidobacterium spp. (54.8%), Bacteroides spp./Parabacteroides spp. (54.8%), Clostridium spp. (35.5%), and Escherichia coli (74.2%) was observed compared with the children without antibiotic therapy (100%, 80%, 63.3%, and 86.6%, respectively). By qPCR, the children receiving antibiotics showed a lower copy number for all microorganisms, except to Lactobacillus spp. (p = 0.0092). In comparison to the nontreated children, the antibiotic-treated children showed a significantly lower copy number of Bifidobacterium spp. (p = 0.0002), Clostridium perfringens (p < 0.0001), E. coli (p = 0.0268), Methanobrevibacter smithii (p = 0.0444), and phylum Firmicutes (p = 0.0009). In conclusion, our results obtained through qualitative and quantitative analyses, demonstrate that antibiotic therapy affect the intestinal microbiome of children. (AU)

• Processo FAPESP: 13/17739-9 - Avaliação clonal de cepas de Clostridium perfringens isolados de humanos e animais por Multilocus sequence typing (MLST)
• Beneficiário: Mario Júlio Ávila-Campos
• Modalidade de apoio: Auxílio à Pesquisa - Regular