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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Alterations of Intestinal Microbiome by Antibiotic Therapy in Hospitalized Children

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Author(s):
Fernandes, Miriam R. ; Ignacio, Aline ; Rodrigues, Viviane A. A. ; Groppo, Franciso C. ; Cardoso, Ary L. ; Avila-Campos, Mario J. ; Nakano, Viviane
Total Authors: 7
Document type: Journal article
Source: MICROBIAL DRUG RESISTANCE; v. 23, n. 1, p. 56+, JAN 2017.
Web of Science Citations: 2
Abstract

The administration of antimicrobial agents leads to an ecological imbalance of the host-microorganisms relationship, and it causes a rapid and significant reduction in the microbial diversity. The aim of the current study was to evaluate the impact of antibiotic therapy on intestinal microbiota of children between 3 and 12 years of age. The fecal samples were collected from hospitalized children (n = 31) and from healthy untreated children (n = 30). The presence of bacteria and their quantities were assessed by culture-based methods and quantitative polymerase chain reaction (qPCR). By culture method, in the children receiving antibiotics, a low recovery of Bifidobacterium spp. (54.8%), Bacteroides spp./Parabacteroides spp. (54.8%), Clostridium spp. (35.5%), and Escherichia coli (74.2%) was observed compared with the children without antibiotic therapy (100%, 80%, 63.3%, and 86.6%, respectively). By qPCR, the children receiving antibiotics showed a lower copy number for all microorganisms, except to Lactobacillus spp. (p = 0.0092). In comparison to the nontreated children, the antibiotic-treated children showed a significantly lower copy number of Bifidobacterium spp. (p = 0.0002), Clostridium perfringens (p < 0.0001), E. coli (p = 0.0268), Methanobrevibacter smithii (p = 0.0444), and phylum Firmicutes (p = 0.0009). In conclusion, our results obtained through qualitative and quantitative analyses, demonstrate that antibiotic therapy affect the intestinal microbiome of children. (AU)

FAPESP's process: 13/17739-9 - Clonal evaluation of Clostridium perfringens strains isolated from humans and animals by Multilocus sequence typing (MLST)
Grantee:Mario Júlio Ávila-Campos
Support Opportunities: Regular Research Grants