Critical Child Microbiota - Evaluation of fecal microbiota of critical children in a University Pediatric Hospital

• Grant number: 15/13059-9
• Support Opportunities: Regular Research Grants
• Start date: October 01, 2015
• End date: March 31, 2018
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Carla Romano Taddei
• Grantee: Carla Romano Taddei
• Host Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated researchers: Gilda Porta ; Rubens Feferbaum

Abstract

The intestinal epithelium of neonates is particularly sensitive area of intestinal mucosa and may have exacerbated inflammatory responses both commensal bacteria and pathogens. Critical newborn admitted in intensive care units are more susceptible to environmental influences, with consequent disorder of the intestinal microbiota and possible proliferation of pathogenic bacteria. Gastroschisis and omphalocele are conditions related to defective closure of the abdominal wall during intrauterine development. Intestinal dismotility or loss antegrade peristalsis complications are often present in these newborns, leading to the use of parenteral nutrition for long periods and the use of broad-spectrum antibiotics, increasing susceptibility to sepsis. Little is known about the composition of the fecal microbiota of critical babies with intestinal wall defects such as gastroschisis and omphalocele and the relationship of premature babies and the appearance of necrotizing enterocolitis. In addition, the primary sclerosing cholangitis (PSC) is a chronic cholestatic liver disease that is characterized by diffuse inflammation, fibrosis and strictures of the biliary ducts intra- and extra-hepatic. The etiology of PSC is still unknown, but there is evidence of the importance of environmental factors such as composition of the intestinal microbiota, and genetic. Thus, the objectives of this study were to assess the evolution of the composition of the intestinal microbiota of severe newborns admitted to the NICU during the hospitalization period and relate any clinical events and evaluate the composition of the fecal microbiota of children with primary sclerosing cholangitis. The results obtained from this project will be important to understand how is the colonization of the intestinal microbiota in critical child and its possible clinical consequences. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CORTEZ, V, RAMON; MOREIRA, LUANA N.; PADILHA, MARINA; BIBAS, MARIANA D.; TOMA, RICARDO K.; PORTA, GILDA; TADDEI, CARLA R.. Gut Microbiome of Children and Adolescents With Primary Sclerosing Cholangitis in Association With Ulcerative Colitis. FRONTIERS IN IMMUNOLOGY, v. 11, FEB 5 2021. (15/13059-9)