Critical Role of Different Immobilized Biocatalysts of a Given Lipase in the Selective Ethanolysis of Sardine Oil

Autor(es)

Moreno-Perez, Sonia ; Machado Turato, Daniela Flavia ; Borges, Janaina Pires ; Luna, Pilar ; Javier Senorans, Francisco ; Guisan, Jose M. ; Fernandez-Lorente, Gloria

• Tipo de documento: Artigo Científico
• Fonte: Journal of Agricultural and Food Chemistry; v. 65, n. 1, p. 117-122, JAN 11 2017.
• Citações Web of Science: 6

Resumo

Different immobilized derivatives of two lipases were tested as catalysts of the synthesis of ethyl esters of omega-3 fatty acids during the ethanolysis of sardine oil in solvent-free systems at 25 degrees C. Lipases from Thermomyces lanuginosus (TLL) and Lecitase Ultra (a phospholipase with lipolytic activity) were studied. Lipases were adsorbed on hydrophobic Sepabeads C18 through the open active center and on an anion-exchanger Duolite with the active center exposed to the reaction medium. TLL-Sepabeads derivatives exhibit a high activity of 9 UI/mg of immobilized enzyme, and they are 20-fold more active than TLL-Duolite derivatives and almost 1000-fold more active than Lipozyme TL IM (the commercial derivative from Novozymes). Lecitase-Sepabeads exhibit a high selectivity for the synthesis of the ethyl ester of EPA that is 43-fold faster than the synthesis of the ethyl ester of DHA. (AU)

• Processo FAPESP: 14/04925-1 - Imobilização de lipase de Penicillium sp J39 por diferentes estratégias, estabilização e caracterização dos derivados
• Beneficiário: Daniela Flavia Machado Turati
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Mestrado