Molecular variability and genetic relationship among Brazilian strains of the sugarcane smut fungus

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Benevenuto, Juliana ; Longatto, Daniel P. ; Reis, Gislaine V. ; Mielnichuk, Natalia ; Palhares, Alessandra C. ; Carvalho, Giselle ; Saito, Suzane ; Quecine, Maria C. ; Sanguino, Alvaro ; Vieira, Maria Lucia C. ; Camargo, Luis Eduardo A. ; Creste, Silvana ; Monteiro-Vitorello, Claudia B.

• Tipo de documento: Artigo Científico
• Fonte: FEMS Microbiology Letters; v. 363, n. 24 DEC 2016.
• Citações Web of Science: 0

Resumo

Sporisorium scitamineum is the fungus that causes sugarcane smut disease. Despite of the importance of sugarcane for Brazilian agribusiness and the persistence of the pathogen in most cropping areas, genetic variation studies are still missing for Brazilian isolates. In this study, sets of isolates were analyzed using two molecular markers (AFLP and telRFLP) and ITS sequencing. Twenty-two whips were collected from symptomatic plants in cultivated sugarcane fields of Brazil. A total of 41 haploid strains of compatible mating types were selected from individual teliospores and used for molecular genetic analyses. telRFLP and ITS analyses were expanded to six Argentine isolates, where the sugarcane smut was first recorded in America. Genetic relationship among strains suggests the human-mediated dispersal of S. scitamineum within the Brazilian territory and between the two neighboring countries. Two genetically distinct groups were defined by the combined analysis of AFLP and telRFLP. The opposite mating-type strains derived from single teliospores were clustered together into these main groups, but had not always identical haplotypes. telRFLP markers analyzed over two generations of selfing and controlled outcrossing confirmed the potential for emergence of new variants and occurrence of recombination, which are relevant events for evolution of virulence and environmental adaptation. (AU)

• Processo FAPESP: 10/05591-9 - Estudos genéticos do fungo Sporosorium scitamineum, agente causador de carvão em cana-de-açúcar
• Beneficiário: Claudia Barros Monteiro Vitorello
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/21802-0 - Especialização ao hospedeiro por fungos causadores do carvão pela perspectiva da genômica comparativa e diversidade genética de isolados de Sporisorium scitamineum (agente causal do carvão na cana-de-açúcar)
• Beneficiário: Juliana Benevenuto
• Modalidade de apoio: Bolsas no Brasil - Doutorado