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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Improved tympanic membrane regeneration after myringoplastic surgery using an artificial biograft

Texto completo
Autor(es):
Immich, A. P. S. ; Pennacchi, P. C. ; Naves, A. F. ; Felisbino, S. L. ; Boemo, R. L. ; Maria-Engler, S. S. ; Catalani, L. H.
Número total de Autores: 7
Tipo de documento: Artigo Científico
Fonte: Materials Science & Engineering C-Materials for Biological Applications; v. 73, p. 48-58, APR 1 2017.
Citações Web of Science: 4
Resumo

Tympanic membrane perforations are due to common otologic problems. The current treatments to heal tympanic membrane perforation, such as myringoplasty, have some disadvantages, including the need for autologous grafting, which is rapidly absorbed by the organism before perforation recovery is complete. To improve the structural and functional tympanic membrane healing after surgery, we propose a new branch of artificial grafts. In this study, we report the development of artificial grafts using electrospun bioabsorbable polymers. Polymers such as poly (L-lactic acid) and poly (lactic-co-glycolic acid) acted as the scaffold for cell growth in a co-culture of fibroblasts and keratinocytes. This co-culture promoted the growth of an epithelial-equivalent tissue over the electrospun scaffold, which was used as an alternative graft in myringoplasty. The in vivo study was performed in Sprague Dawley rats. Ear endoscopy was performed 30 days after surgery and showed that tympanic membrane perforations treated with artificial grafts healed naturally, completely and with the possibility of maintaining their actual functionality. In conclusion, our study described a new artificial graft created specifically to fulfill the requirements of perforated tympanic membrane healing processes, which are compatibility, proper durability and less intense side effects following myringoplasty. (C) 2016 Elsevier B.V. All rights reserved. (AU)

Processo FAPESP: 11/18017-1 - Desenvolvimento de matrizes poliméricas eletrofiadas para regeneração de membranas timpânicas perfuradas
Beneficiário:Ana Paula Serafini Immich
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 11/22812-1 - Desenvolvimento de pele artificial contendo equivalente dérmico glicado mimetizando a fisiopatologia da pele envelhecida e diabética
Beneficiário:Paula Comune Pennacchi
Linha de fomento: Bolsas no Brasil - Doutorado
Processo FAPESP: 11/21442-6 - Polímeros sintéticos e naturais aplicados à engenharia de tecido
Beneficiário:Luiz Henrique Catalani
Linha de fomento: Auxílio à Pesquisa - Temático