Ethylene-induced transcriptional and hormonal responses at the onset of sugarcane ripening

Autor(es)

Cunha, Camila P. ; Roberto, Guilherme G. ; Vicentini, Renato ; Lembke, Carolina G. ; Souza, Glaucia M. ; Ribeiro, Rafael V. ; Machado, Eduardo C. ; Lagoa, Ana M. M. A. ; Menossi, Marcelo

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 7, MAR 7 2017.
• Citações Web of Science: 14

Resumo

The effects of ethephon as a sugarcane ripener are attributed to ethylene. However, the role of this phytohormone at the molecular level is unknown. We performed a transcriptome analysis combined with the evaluation of sucrose metabolism and hormone profiling of sugarcane plants sprayed with ethephon or aminoethoxyvinylglycine (AVG), an ethylene inhibitor, at the onset of ripening. The differential response between ethephon and AVG on sucrose level and sucrose synthase activity in internodes indicates ethylene as a potential regulator of sink strength. The correlation between hormone levels and transcriptional changes suggests ethylene as a trigger of multiple hormone signal cascades, with approximately 18% of differentially expressed genes involved in hormone biosynthesis, metabolism, signalling, and response. A defence response elicited in leaves favoured salicylic acid over the ethylene/jasmonic acid pathway, while the upper internode was prone to respond to ethylene with strong stimuli on ethylene biosynthesis and signalling genes. Besides, ethylene acted synergistically with abscisic acid, another ripening factor, and antagonistically with gibberellin and auxin. We identified potential ethylene target genes and characterized the hormonal status during ripening, providing insights into the action of ethylene at the site of sucrose accumulation. A molecular model of ethylene interplay with other hormones is proposed. (AU)

• Processo FAPESP: 08/57495-3 - Integrando características fisiológicas, morfológicas e anatômicas para entender o rendimento diferencial de sacarose em genótipos de cana-de-açúcar
• Beneficiário: Eduardo Caruso Machado
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Temático
• Processo FAPESP: 08/54201-9 - Identificação e caracterização de promotores de cana-de-açúcar induzidos por seca
• Beneficiário: Carolina Gimiliani Lembke
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 12/13920-8 - Análise transcriptômica do efeito do hormônio etileno na maturação em cana-de-açúcar
• Beneficiário: Camila Pinto da Cunha
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 13/15576-5 - Abordagens moleculares para estudar a relação entre etileno e maturação em cana-de-açúcar
• Beneficiário: Marcelo Menossi Teixeira
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Temático
• Processo FAPESP: 08/52146-0 - Sugarcane signaling and regulatory networks
• Beneficiário: Glaucia Mendes Souza
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOEN - Temático