| Full text | |
| Author(s): |
Cunha, Camila P.
;
Roberto, Guilherme G.
;
Vicentini, Renato
;
Lembke, Carolina G.
;
Souza, Glaucia M.
;
Ribeiro, Rafael V.
;
Machado, Eduardo C.
;
Lagoa, Ana M. M. A.
;
Menossi, Marcelo
Total Authors: 9
|
| Document type: | Journal article |
| Source: | SCIENTIFIC REPORTS; v. 7, MAR 7 2017. |
| Web of Science Citations: | 14 |
| Abstract | |
The effects of ethephon as a sugarcane ripener are attributed to ethylene. However, the role of this phytohormone at the molecular level is unknown. We performed a transcriptome analysis combined with the evaluation of sucrose metabolism and hormone profiling of sugarcane plants sprayed with ethephon or aminoethoxyvinylglycine (AVG), an ethylene inhibitor, at the onset of ripening. The differential response between ethephon and AVG on sucrose level and sucrose synthase activity in internodes indicates ethylene as a potential regulator of sink strength. The correlation between hormone levels and transcriptional changes suggests ethylene as a trigger of multiple hormone signal cascades, with approximately 18% of differentially expressed genes involved in hormone biosynthesis, metabolism, signalling, and response. A defence response elicited in leaves favoured salicylic acid over the ethylene/jasmonic acid pathway, while the upper internode was prone to respond to ethylene with strong stimuli on ethylene biosynthesis and signalling genes. Besides, ethylene acted synergistically with abscisic acid, another ripening factor, and antagonistically with gibberellin and auxin. We identified potential ethylene target genes and characterized the hormonal status during ripening, providing insights into the action of ethylene at the site of sucrose accumulation. A molecular model of ethylene interplay with other hormones is proposed. (AU) | |
| FAPESP's process: | 08/57495-3 - Integrating physiological, morphological and anatomical traits to understand the differential sucrose yield in sugarcane genotypes |
| Grantee: | Eduardo Caruso Machado |
| Support Opportunities: | Program for Research on Bioenergy (BIOEN) - Thematic Grants |
| FAPESP's process: | 08/54201-9 - Identification and characterization of sugarcane promoters induced by drought |
| Grantee: | Carolina Gimiliani Lembke |
| Support Opportunities: | Scholarships in Brazil - Doctorate (Direct) |
| FAPESP's process: | 12/13920-8 - TRANSCRIPTOMIC ANALYSIS OF THE EFFECT OF HORMONE ETHYLENE IN SUGARCANE RIPENING |
| Grantee: | Camila Pinto da Cunha |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 13/15576-5 - Molecular approaches to study the relationship between ethylene and sugarcane ripening |
| Grantee: | Marcelo Menossi Teixeira |
| Support Opportunities: | Program for Research on Bioenergy (BIOEN) - Thematic Grants |
| FAPESP's process: | 08/52146-0 - Sugarcane signaling and regulatory networks |
| Grantee: | Glaucia Mendes Souza |
| Support Opportunities: | Program for Research on Bioenergy (BIOEN) - Thematic Grants |