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Cross-talk between brassinosteroid and ethylene in sugarcane ripening: regulation of ACC synthase via brassinosteroid

Grant number: 14/10003-0
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): December 01, 2014
Effective date (End): January 31, 2019
Field of knowledge:Biological Sciences - Genetics - Plant Genetics
Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal researcher:Marcelo Menossi Teixeira
Grantee:José Sérgio de Macedo Soares
Home Institution: Instituto de Biologia (IB). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
Associated research grant:13/15576-5 - Molecular approaches to study the relationship between ethylene and sugarcane ripening, AP.BIOEN.TEM
Associated scholarship(s):15/25469-7 - Regulation of ACC synthase in response to brassinosteroid, BE.EP.PD

Abstract

Sugarcane is a C4 plant that has a unique capacity to accumulate high concentrations of sucrose in the stem. Ethylene is phytohormone that plays an important role on regulation of growth and sucrose accumulation of sugarcane. The rate limiting step of ethylene biosynthesis is mediated by the enzyme 1-aminocyclopropane-1-carboxylase synthase (ACS). In several hormone biosynthesis and signaling pathways, protein turnover has emerged as a common regulatory element. These proteins may function as cross-talk points, interconnecting hormone signaling pathway with molecular mechanisms regulating various aspects of plant physiology. Brassinosteroid (BR) is steroid hormone and function as a master regulator of plant growth and belong to a group of factors influencing ethylene biosynthesis through regulation of ACS protein stability. In this research project we will investigate the effects of brassinosteroid in ethylene biosynthesis via sugarcane ACS regulation. Initially, we will evaluate if BRs induce the ethylene biosynthesis in sugarcane seedlings. To expand the comprehension of the BR signaling components involved in ethylene biosynthesis we will measure ethylene emission in rice loss-of-function BR signaling mutants. To complement this assay we also expect to identify a physical interaction between sugarcane proteins homologous to BR signaling proteins (ScBRI1, ScBAK1 or ScBZR1) and three different sugarcane ACS isoforms via bimolecular fluorescence complementation using rice protoplasts. And to determine if ethylene production by BR is the result of an increase in ACSs stability in vivo we will measure the levels of fusion proteins in transgenic rice seedlings expressing sugarcane myc-epitope-tagged ACS under the control of the dexamethasone inducible promoter in the presence of BR. The elucidation of ACS regulation mechanism via brassinosteroid signaling will provide significant knowledge not only in sugarcane growth and ripening, but also for plant in general. (AU)

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