Laminin-1 and SIKVAV a laminin-1-derived peptide, regulate the morphology and protease activity of a human salivary gland adenoid cystic carcinoma cell line

Autor(es)

Freitas, V. M. ^[1] ; Scheremeta, B. ; Hoffman, M. P. ; Jaeger, R. G.

Afiliação do(s) autor(es)

^[1] Universidade de São Paulo (USP). Instituto de Ciências Biomédicas. Departamento de Biologia Celular e do Desenvolvimento - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Oral Oncology; v. 40, n. 5, p. 483-489, maio 2004.
• Área do conhecimento: Ciências da Saúde - Odontologia
• Assunto(s): Neoplasias das glândulas salivares Metaloproteinases Matriz extracelular Peptídeos

Resumo

In a previous paper, we demonstrated that laminin-1 and its derived peptide SIKVAV modulates the morphology of an adenoid cystic carcinoma cell line (CAC2 cells). Light microscopy of CAC2 cells grown in three-dimensional preparations of SIKVAV-enriched laminin-1 showed the presence of pseudocystic spaces. Pseudocysts are hallmarks of adenoid cystic carcinoma in vivo. We hypothesized that these pseudocystic spaces could be due to the protease-inducing/activating role of SIKVAV. Thus, we studied the presence of matrix metalloproteinases (MMPs) in CAC2 cells treated either by laminin-1 or by SIKVAV-enriched laminin-1. Immunohistochemistry and zymography suggested that SIKVAV enhanced the secretion of MMP-2 and MMP-9 in CAC2 cells. We propose that SIKVAV induces pseudocystic formation probably through the secretion of MMPs 2 and 9. (AU)

• Processo FAPESP: 00/04693-0 - Efeito da laminina e de alguns de seus peptídeos bioativos, no fenótipo de linhagens celulares derivadas de adenoma pleomórfico, mioepitelioma e carcinoma adenóide cístico humano: estudo morfológico, imunohistoquímico e subcelular
• Beneficiário: Ruy Gastaldoni Jaeger
• Modalidade de apoio: Auxílio à Pesquisa - Regular