| Full text | |
| Author(s): |
Freitas, V. M.
[1]
;
Scheremeta, B.
;
Hoffman, M. P.
;
Jaeger, R. G.
Total Authors: 4
|
| Affiliation: | [1] Universidade de São Paulo (USP). Instituto de Ciências Biomédicas. Departamento de Biologia Celular e do Desenvolvimento - Brasil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | Oral Oncology; v. 40, n. 5, p. 483-489, maio 2004. |
| Field of knowledge: | Health Sciences - Dentistry |
| Abstract | |
In a previous paper, we demonstrated that laminin-1 and its derived peptide SIKVAV modulates the morphology of an adenoid cystic carcinoma cell line (CAC2 cells). Light microscopy of CAC2 cells grown in three-dimensional preparations of SIKVAV-enriched laminin-1 showed the presence of pseudocystic spaces. Pseudocysts are hallmarks of adenoid cystic carcinoma in vivo. We hypothesized that these pseudocystic spaces could be due to the protease-inducing/activating role of SIKVAV. Thus, we studied the presence of matrix metalloproteinases (MMPs) in CAC2 cells treated either by laminin-1 or by SIKVAV-enriched laminin-1. Immunohistochemistry and zymography suggested that SIKVAV enhanced the secretion of MMP-2 and MMP-9 in CAC2 cells. We propose that SIKVAV induces pseudocystic formation probably through the secretion of MMPs 2 and 9. (AU) | |