Role of tyrosol on Candida albicans, Candida glabrata and Streptococcus mutans biofilms developed on different surfaces

Autor(es)

Monteiro, Douglas Roberto ; Arias, Lais Salomao ; Fernandes, Renan Aparecido ; Straioto, Fabiana Gouveia ; Barbosa, Debora Barros ; Pessan, Juliano Pelim ; Botazzo Delbem, Alberto Carlos

• Tipo de documento: Artigo Científico
• Fonte: AMERICAN JOURNAL OF DENTISTRY; v. 30, n. 1, p. 35-39, FEB 2017.
• Citações Web of Science: 2

Resumo

Purpose: To assess the effect of tyrosol on the production of hydrolytic enzymes (by Candida biofilm cells) and acid (by Streptococcus mutans biofilms), as well as to quantify single and mixed biofilms of these species formed on acrylic resin (AR) and hydroxyapatite (HA). Methods: Candida and S. mutans biofilms were formed on AR and HA in the presence of tyrosol during 48 hours. Next, acid proteinase, phospholipase and hemolytic activities of Candida biofilm cells were determined, while acid production by S. mutans biofilms was assessed by pH determination. The effect of tyrosol on mature biofilms (96 hours) was evaluated through quantification of total biomass, metabolic activity, number of colony-forming units and composition of biofilms' extracellular matrix. Data were analyzed by one and two-way ANOVA, followed by Tukey's and Holm-Sidak's tests (alpha = 0.05). Results: Treatments with tyrosol were not able to significantly reduce hydrolytic enzymes and acid production by Candida and S. mutans. Tyrosol only significantly reduced the metabolic activity of single biofilms of Candida species. (AU)

• Processo FAPESP: 13/10285-2 - Efeito do tirosol sobre fatores de virulência de Candida albicans, Candida glabrata e Streptococcus mutans
• Beneficiário: Alberto Carlos Botazzo Delbem
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/05507-9 - Efeito do Tirosol na produção de ácido láctico e na capacidade de formação de biofilmes por Streptococcus mutans
• Beneficiário: Laís Salomão Arias
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 13/03273-8 - Efeito do Tirosol sobre fatores de virulência de Candida albicans, Candida glabrata e Streptococcus mutans
• Beneficiário: Douglas Roberto Monteiro
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado