Virulence-related genes, adhesion and invasion of some Yersinia enterocolitica-like strains suggests its pathogenic potential

Autor(es)

Imori, Priscilla F. M. ; Passaglia, Jaqueline ; Souza, Roberto A. ; Rocha, Lenaldo B. ; Falcao, Juliana P.

• Tipo de documento: Artigo Científico
• Fonte: Microbial Pathogenesis; v. 104, p. 72-77, MAR 2017.
• Citações Web of Science: 4

Resumo

Yersina enterocolitica-like species have not been extensively studied regarding its pathogenic potential. This work aimed to assess the pathogenic potential of some Y. enterocolitica-like strains by evaluating the presence of virulence-related genes by PCR and their ability to adhere to and invade Caco-2 and HEp-2 cells. A total of 50 Y. frederiksenii, 55 Y. intermedia and 13 Y. kristensenii strains were studied. The strains contained the following genes: Y. frederiksenii, fepA(44%), fes(44%) and ystB(18%); Y. intermedia, ail(53%), fepA (35%), fepD(2%), fes(97%), hreP(2%), ystB(2%) and tccC(35%); Y. kristensenii, ail(62%), ystB(23%), fepA(77%), fepD(54%), fes(54%) and hreP(77%). Generally, the Y. enterocolitica-like strains had a reduced ability to adhere to and invade mammalian cells compared to the highly pathogenic Y. enterocolitica 8081. However, Y. kristensenii FCF410 and Y frederiksenii FCF461 presented high invasion potentials in Caco-2 cells after five days of pre-incubation increased by 45- and 7.2-fold compared to Y. enterocolitica 8081, respectively; but, the ail gene was not detected in these strains. The presence of virulence-related genes in some of the Y. enterocolitica-like strains indicated their possible pathogenic potential. Moreover, the results suggest the existence of alternative virulence mechanisms and that the pathogenicity of Y. kristensenii and Y. frederiksenii may be strain-dependent. (C) 2017 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 10/19830-5 - Genotipagem de linhagens de Yersinia spp. por high-resolution melting analysis
• Beneficiário: Juliana Pfrimer Falcão
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 09/08455-1 - Genotipagem de linhagens de Yersinia ssp. por high-resolution melting analysis
• Beneficiário: Roberto Antonio de Souza
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 12/19132-1 - Caracterização molecular de linhagens de Salmonella Typhimurium isoladas de humanos, alimentos e animais no Sudeste e Sul do Brasil
• Beneficiário: Juliana Pfrimer Falcão
• Modalidade de apoio: Auxílio à Pesquisa - Regular