Protein Oligomerization Based on Bronsted Acid Reaction

Autor(es)

Pereira, Andressa R. ; Luz, Roberto A. S. ; Lima, Filipe C. D. A. ; Crespilho, Frank N.

• Tipo de documento: Artigo Científico
• Fonte: ACS CATALYSIS; v. 7, n. 4, p. 3082-3088, APR 2017.
• Citações Web of Science: 2

Resumo

Oligomeric proteins are abundant in nature. However, their synthesis remains a significant challenge. A controlled oligomerization process can provide important insights into the evolution of modern proteins and the development of more efficient biocatalysts. Here, we propose a pathway for producing glucose oxidase (GOx) homooligomer (Ol-GOx), a redox enzyme with extensive biotechnological applications. We obtained Ol-GOx from the one-pot reaction of the native protein with a Bronsted acid, trifluoromethanesulfonic acid (TFMS). Ol-GOx had a hydrodynamic radius of 96 nm and a molecular mass of 2 MDa. Ol-GOx exhibited higher thermal stability in comparison to the native protein, as well as increased hydrophobicity, which is a primary characteristic needed for its stabilization in the solid state for applications in bioenergy, heterogeneous catalysis, and biomedicine. Furthermore, there were remarkable improvements in redox properties and protein stabilization, suggesting that this approach for Ol-GOx synthesis could be used to design more efficient oligomeric biocatalysts. (AU)

• Processo FAPESP: 15/16672-3 - Desenvolvimento de Bioeletrodos de Alto Desempenho para Miniaturização de Biocélulas a Combustível
• Beneficiário: Frank Nelson Crespilho
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/19908-2 - Estudo bioeletroquímico da enzima glicose oxidase e glicose oxidase deglicosilada para aplicação em biocélulas a combustível
• Beneficiário: Andressa Ribeiro Pereira
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 13/14262-7 - Filmes nanoestruturados de materiais de interesse biológico
• Beneficiário: Osvaldo Novais de Oliveira Junior
• Modalidade de apoio: Auxílio à Pesquisa - Temático