| Texto completo | |
| Autor(es): |
Ezra-Elia, Raaya
;
da Silva, Germana Alegro
;
Zanoni, Diogo Sousa
;
Laufer-Amorim, Renee
;
Couto do Amaral, Andreia Vitor
;
Laus, Jose Luiz
;
Ofri, Ron
Número total de Autores: 7
|
| Tipo de documento: | Artigo Científico |
| Fonte: | CURRENT EYE RESEARCH; v. 42, n. 3, p. 452-461, 2017. |
| Citações Web of Science: | 1 |
| Resumo | |
Purpose: Retinal ischemia is a common cause of visual impairment and blindness. Sildenafil, a PDE5 inhibitor which inhibits cGMP degradation and in turn prolongs the effect of nitric oxide, has been shown to be protective in a number of ischemia/reperfusion (I/R) injuries, as well as in neuronal damage. We hypothesized that treatment with sildenafil might be neuroprotective in a model of acute retinal I/R injury.Methods: Anterior chamber cannulation was performed to induce unilateral I/R injury in 38 Lewis rats. Animals received intraperitoneal injections of sildenafil (0.5 and 1 mg/kg once a day, for a period of 7 and 18 days, respectively), or saline. Electroretinography recordings, retinal ganglion cell (RGC) counts following retrograde labeling with fluorogold, histopathology, and immunohistochemistry (IHC) using antibodies against PDE5, NOS2, caspase-3, caspase-9, and Bcl-2 were conducted.Results: No significant differences in electroretinography, RGC counts, or retinal morphometry were observed between experimental eyes of sildenafil- and saline-treated animals. A tendency toward less necrosis in histopathology, and a slight trend toward lower PDE5, NOS2, and caspase-9 and higher Bcl-2 IHC scores were evident in experimental retinas of sildenafil-treated animals.Conclusions: Electroretinography, RGC counts, and retinal morphometry failed to show any neuroprotective effect of sildenafil in acute retinal I/R injury in rats. A slight positive effect of sildenafil was qualitatively indicated by histopathology and IHC. (AU) | |
| Processo FAPESP: | 11/51269-4 - Preserving vision in glaucoma: preventing optic neuropathy through neuroprotective, vasodilative treatment |
| Beneficiário: | José Luiz Laus |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |