Linkage and mapping of quantitative trait loci associated with angular leaf spot and powdery mildew resistance in common beans

Autor(es):Mostrar menos -

Denis Bassi ^[1] ; Boris Briñez ^[2] ; Juliana Santa Rosa ^[3] ; Paula Rodrigues Oblessuc ^[4] ; Caléo Panhoca de Almeida ^[5] ; Stella Maris Nucci ^[6] ; Larissa Chariel Domingos da Silva ^[7] ; Alisson Fernando Chiorato ^[8] ; Rosana Pereira Vianello ^[9] ; Luis Eduardo Aranha Camargo ^[10] ; Matthew Wohlgemuth Blair ^[11] ; Luciana Lasry Benchimol-Reis ^[12]

Afiliação do(s) autor(es):Mostrar menos -

^[1] Instituto Agronômico de Campinas. Centro de Recursos Genéticos Vegetais - Brasil

^[2] Instituto Agronômico de Campinas. Centro de Recursos Genéticos Vegetais - Brasil

^[3] Instituto Agronômico de Campinas. Centro de Recursos Genéticos Vegetais - Brasil

^[4] Instituto Agronômico de Campinas. Centro de Recursos Genéticos Vegetais - Brasil

^[5] Instituto Agronômico de Campinas. Centro de Recursos Genéticos Vegetais - Brasil

^[6] Instituto Agronômico de Campinas. Centro de Recursos Genéticos Vegetais - Brasil

^[7] Instituto Agronômico de Campinas. Centro de Recursos Genéticos Vegetais - Brasil

^[8] Instituto Agronômico de Campinas. Centro de Grãos e Fibras - Brasil

^[9] EMBRAPA. Centro Nacional de Pesquisa de Arroz e Feijão - Brasil

^[10] Universidade de São Paulo. Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Departamento de Fitopatologia - Brasil

^[11] Tennessee State University. Department of Agriculture and Environmental Sciences - Estados Unidos

^[12] Instituto Agronômico de Campinas. Centro de Recursos Genéticos Vegetais - Brasil

• Tipo de documento: Artigo Científico
• Fonte: GENETICS AND MOLECULAR BIOLOGY; v. 40, n. 1, p. 109-122, 2017-02-20.

Resumo

Abstract Angular leaf spot (ALS) and powdery mildew (PWM) are two important fungi diseases causing significant yield losses in common beans. In this study, a new genetic linkage map was constructed using single sequence repeats (SSRs) and single nucleotide polymorphisms (SNPs), in a segregating population derived from the AND 277 x SEA 5 cross, with 105 recombinant inbred lines. Phenotypic evaluations were performed in the greenhouse to identify quantitative trait loci (QTLs) associated with resistance by means of the composite interval mapping analysis. Four QTLs were identified for ALS resistance. The QTL ALS11AS, linked on the SNP BAR 5054, mapped on chromosome Pv11, showed the greatest effect (R2 = 26.5%) on ALS phenotypic variance. For PWM resistance, two QTLs were detected, PWM2AS and PWM11AS, on Pv2 and Pv11, explaining 7% and 66% of the phenotypic variation, respectively. Both QTLs on Pv11 were mapped on the same genomic region, suggesting that it is a pleiotropic region. The present study resulted in the identification of new markers closely linked to ALS and PWM QTLs, which can be used for marker-assisted selection, fine mapping and positional cloning. (AU)

• Processo FAPESP: 10/51673-7 - Identificação de genes candidatos para resistência à mancha angular em feijão comum (Phaseolus vulgaris L.)
• Beneficiário: Luciana Lasry Benchimol-Reis
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/51186-9 - Mapeamento de locos associados a tolerancia ao estresse hidrico com a plataforma dart e marcadores microssatelites.
• Beneficiário: Luciana Lasry Benchimol-Reis
• Modalidade de apoio: Auxílio à Pesquisa - Regular