Improving Sample Distribution Homogeneity in Three-Dimensional Microfluidic Paper-Based Analytical Devices by Rational Device Design

Autor(es)

Morbioli, Giorgio Gianini ; Mazzu-Nascimento, Thiago ; Milan, Luis Aparecido ; Stockton, Amanda M. ; Carrilho, Emanuel

• Tipo de documento: Artigo Científico
• Fonte: Analytical Chemistry; v. 89, n. 9, p. 4786-4792, MAY 2 2017.
• Citações Web of Science: 23

Resumo

Paper-based devices are a portable, user-friendly, and affordable technology that is one of the best analytical tools for inexpensive diagnostic devices. Three-dimensional microfluidic paper-based analytical devices (3D-mu PADs) are an evolution of single layer devices and they permit effective sample dispersion, individual layer treatment, and multiplex analytical assays. Here, we present the rational design of a wax-printed 3D-mu PAD that enables more homogeneous permeation of fluids along the cellulose matrix than other existing designs in the literature. Moreover, we show the importance of the rational design of channels on these devices using glucose oxidase, peroxidase, and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) reactions. We present an alternative method for layer stacking using a magnetic apparatus, which facilitates fluidic dispersion and improves the reproducibility of tests performed on 3D-mu PADs. We also provide the optimized designs for printing, facilitating further studies using 3D-mu PADs. (AU)

• Processo FAPESP: 11/13997-8 - Plataformas em papel para detecção não invasiva de biomarcadores tumorais e para cultivo de células neoplásicas
• Beneficiário: Thiago Mazzu Do Nascimento
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto
• Processo FAPESP: 08/57805-2 - Instituto de Bioanalítica
• Beneficiário: Lauro Tatsuo Kubota
• Modalidade de apoio: Auxílio à Pesquisa - Temático