Improving Sample Distribution Homogeneity in Three-Dimensional Microfluidic Paper-Based Analytical Devices by Rational Device Design

Author(s)

Morbioli, Giorgio Gianini ; Mazzu-Nascimento, Thiago ; Milan, Luis Aparecido ; Stockton, Amanda M. ; Carrilho, Emanuel

• Document type: Journal article
• Source: Analytical Chemistry; v. 89, n. 9, p. 4786-4792, MAY 2 2017.
• Web of Science Citations: 23

Abstract

Paper-based devices are a portable, user-friendly, and affordable technology that is one of the best analytical tools for inexpensive diagnostic devices. Three-dimensional microfluidic paper-based analytical devices (3D-mu PADs) are an evolution of single layer devices and they permit effective sample dispersion, individual layer treatment, and multiplex analytical assays. Here, we present the rational design of a wax-printed 3D-mu PAD that enables more homogeneous permeation of fluids along the cellulose matrix than other existing designs in the literature. Moreover, we show the importance of the rational design of channels on these devices using glucose oxidase, peroxidase, and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) reactions. We present an alternative method for layer stacking using a magnetic apparatus, which facilitates fluidic dispersion and improves the reproducibility of tests performed on 3D-mu PADs. We also provide the optimized designs for printing, facilitating further studies using 3D-mu PADs. (AU)

• FAPESP's process: 11/13997-8 - Paper-based platforms for noninvasive detection of tumor biomarkers and cultive neoplastic cells
• Grantee: Thiago Mazzu Do Nascimento
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 08/57805-2 - Institute of Bioanalytics
• Grantee: Lauro Tatsuo Kubota
• Support Opportunities: Research Projects - Thematic Grants