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Microfluidic devices with electrochemical detection for the extraction and detection of compounds of biological interest

Grant number: 13/24255-8
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): March 01, 2014
Effective date (End): March 31, 2015
Field of knowledge:Physical Sciences and Mathematics - Chemistry
Principal Investigator:Lauro Tatsuo Kubota
Grantee:Murilo Santhiago
Home Institution: Instituto de Química (IQ). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

The main goal of this research project is the development of microfluidic devices based on paper with an integrated electrochemical detection system for the analysis of lactate. The choice of paper as the platform for microchannels construction is due to the numerous advantages of this porous material for the fabrication of microfluidic devices. The microchannels will be fabricated on paper using electronic cutting/graving tools. The silanization step will be studied in order to obtain hydrophobic walls and also expose functional groups that allow enzyme immobilization. For this reason, it will be studied here a mixed silanization in both gas and liquid phases. The sealing process and coupling of the electrochemical detection system will be conducted in the hydrophobyzed microchannels. Low cost microelectrodes will be integrated with the microchannels and used for lactate detection at low applied potentials. The extraction process of the analyte from filter papers used for the collection of biological samples will be monitored by chronoamperometry.