Transdentinal photobiostimulation of stem cells from human exfoliated primary teeth

Autor(es)

Turrioni, A. P. ; Basso, F. G. ; Montoro, L. A. ; Almeida, L. F. D. ; de Souza Costa, C. A. ; Hebling, J.

• Tipo de documento: Artigo Científico
• Fonte: International Endodontic Journal; v. 50, n. 6, p. 549-559, JUN 2017.
• Citações Web of Science: 1

Resumo

AimTo evaluate the effects of infrared light-emitting diode (LED) irradiation on stem cells from human exfoliated deciduous teeth (SHEDs). MethodologyExfoliated primary teeth were obtained (n=3), and SHEDs obtained from the teeth were seeded on the pulpal surface of 0.2-mm-thick dentine discs produced from permanent molars. The cells were incubated for 24h by placing the discs in plain Dulbecco's modified Eagle's medium (DMEM). The DMEM was then replaced with new culture medium formulated for odontoblast differentiation. After 12h in the second medium, SHEDs were irradiated through the dentine discs using an infrared LED (850nm) with a power density of 80mWcm(-2). Energy doses (EDs) delivered to the occlusal surface of the dentine discs were 0 (control), 2 and 4Jcm(-2) (n=6). Subsequent tests were performed 72h after irradiation. These tests included cell viability (MTT), alkaline phosphatase activity (ALP), total protein production (TP), scanning electron microscopy (SEM), as well as gene expression for ALP, Col I, DSPP and DMP-1. Data were analysed using Kruskal-Wallis and Mann-Whitney t-tests (=0.05). ResultsBoth EDs (2 and 4Jcm(-2)) significantly increased cell viability and ALP activity. For TP, ALP and Col I gene expression, only the 4Jcm(-2) group had significantly higher values compared to the control group. Cell morphology was not affected by irradiation. ConclusionInfrared LED irradiation was capable of biostimulating SHEDs through a 0.2mm thickness of dentine, especially at the 4Jcm(-2) level. (AU)

• Processo FAPESP: 11/13895-0 - Efeito direto e transdentinário do LED infravermelho sobre células-tronco da polpa dentária humana in vitro e sobre o complexo dentino-pulpar in vivo
• Beneficiário: Ana Paula Silveira Turrioni
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 13/17758-3 - Efeito da técnica de isolamento, fonte do tecido e passagem celular no número e fenótipo de células-tronco originárias da polpa dentária
• Beneficiário: Ana Paula Silveira Turrioni
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado