| Full text | |
| Author(s): |
Turrioni, A. P.
;
Basso, F. G.
;
Montoro, L. A.
;
Almeida, L. F. D.
;
de Souza Costa, C. A.
;
Hebling, J.
Total Authors: 6
|
| Document type: | Journal article |
| Source: | International Endodontic Journal; v. 50, n. 6, p. 549-559, JUN 2017. |
| Web of Science Citations: | 1 |
| Abstract | |
AimTo evaluate the effects of infrared light-emitting diode (LED) irradiation on stem cells from human exfoliated deciduous teeth (SHEDs). MethodologyExfoliated primary teeth were obtained (n=3), and SHEDs obtained from the teeth were seeded on the pulpal surface of 0.2-mm-thick dentine discs produced from permanent molars. The cells were incubated for 24h by placing the discs in plain Dulbecco's modified Eagle's medium (DMEM). The DMEM was then replaced with new culture medium formulated for odontoblast differentiation. After 12h in the second medium, SHEDs were irradiated through the dentine discs using an infrared LED (850nm) with a power density of 80mWcm(-2). Energy doses (EDs) delivered to the occlusal surface of the dentine discs were 0 (control), 2 and 4Jcm(-2) (n=6). Subsequent tests were performed 72h after irradiation. These tests included cell viability (MTT), alkaline phosphatase activity (ALP), total protein production (TP), scanning electron microscopy (SEM), as well as gene expression for ALP, Col I, DSPP and DMP-1. Data were analysed using Kruskal-Wallis and Mann-Whitney t-tests (=0.05). ResultsBoth EDs (2 and 4Jcm(-2)) significantly increased cell viability and ALP activity. For TP, ALP and Col I gene expression, only the 4Jcm(-2) group had significantly higher values compared to the control group. Cell morphology was not affected by irradiation. ConclusionInfrared LED irradiation was capable of biostimulating SHEDs through a 0.2mm thickness of dentine, especially at the 4Jcm(-2) level. (AU) | |
| FAPESP's process: | 11/13895-0 - Transdentinal and direct effect of infrared LED on human dental pulp cells and pulp-dentin complex |
| Grantee: | Ana Paula Silveira Turrioni |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 13/17758-3 - Effect of the isolation technique, source of tissue and cell passage on the number and phenotype of stem cells originating from dental pulp |
| Grantee: | Ana Paula Silveira Turrioni |
| Support Opportunities: | Scholarships abroad - Research Internship - Doctorate |