Equine herpesvirus type 1 induces both neurological and respiratory disease in Syrian hamsters

Autor(es)

Mesquita, Leonardo Pereira ; Arevalo, Andressa Ferrari ; Zanatto, Dennis A. ; Miyashiro, Samantha Iye ; Sequetin Cunha, Elenice Maria ; Custodio de Souza, Maria do Carmo ; Cassaro Villalobos, Eliana Monteforte ; Cabrera Mori, Claudia Madalena ; Maiorka, Paulo Cesar ; Mori, Enio

• Tipo de documento: Artigo Científico
• Fonte: Veterinary Microbiology; v. 203, p. 117-124, MAY 2017.
• Citações Web of Science: 1

Resumo

The equine herpesvirus type 1 (EHV-1) is an important cause of myeloencephalopathy and respiratory disease in horses. Animal models for EHV-1 infection have been specially developed using mice and Syrian hamsters (Mesocricetus auratus). However, few studies have attempted to evaluate the pathogenesis of EHV-1 infection in the central nervous system (CNS) and respiratory system of hamsters. Therefore, the aim of this study was to evaluate the pathogenesis of four Brazilian EHV-1 strains within the CNS and lungs of Syrian hamsters. Hamsters intranasally infected with A4/72, A9/92, A3/97, and Iso/72 EHV-1 strains developed severe neurological and respiratory signs and died during acute EHV-1 infection within 3 to 5 days post-inoculation. However, neurological signs were more severe in A4/72 and A9/92-infected hamsters, whereas respiratory signs were more prominent in A3/97 and Iso/72-infected hamsters. In the latter, lesions in the CNS were predominantly inflammatory, whereas in A4/72 and A9/92-infected hamsters, neuronal and liquefactive necrosis were the predominant lesions. EHV-1 infected hamsters also developed an interstitial pneumonia with infiltration of alveolar septa by macrophages, neutrophils, and lymphocytes, with the exception of A9/92-infected hamsters, which developed severe hemorrhages within the airways. EHV-1 antigens were detected along with CNS and pulmonary lesions. EHV-1 was also recovered from CNS of all infected hamsters, whereas the virus was recovered from the lungs of A4/72, A9/92, and Iso/72-infected hamsters. Brazilian EHV-1 strains caused both severe CNS and respiratory disease in hamsters, thus making this species an interesting model for EHV-1 infection in the CNS and respiratory system. (C) 2017 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 12/24769-9 - Identificação de marcadores da resposta imune e inflamatória em encefalites virais induzidas experimentalmente em um modelo murino
• Beneficiário: Paulo César Maiorka
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/09396-2 - Caracterização da resposta imune no sistema nervoso central de camundongos infectados por estirpes altamente neurovirulentas de Herpesvírus Equino tipo 1
• Beneficiário: Leonardo Pereira Mesquita
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado