Response of digestive organs of Hypsiboas albopunctatus (Anura: Hylidae) to benzo[alpha]pyrene

Autor(es)

Fanali, Lara Zacari ; de lacerda Valverde, Bruno Serra ; Franco-Belussi, Lilian ; Provete, Diogo B. ; de Oliveira, Classius

• Tipo de documento: Artigo Científico
• Fonte: Amphibia-Reptilia; v. 38, n. 2, p. 175-185, 2017.
• Citações Web of Science: 1

Resumo

Anurans are exposed to several pollutants. One of these is benzo{[}alpha]pyrene (BaP). This compound is produced by incomplete combustion and is toxic to the liver and intestine, where it is metabolized. Here, we tested how different concentrations of BaP affect the thickness of small intestine and liver melanomacrophages (MMCs) of Hypsiboas albopunctatus during short-and long-term exposures. We conducted an experiment with a 3 x 2 factorial design to answer these two questions. Male specimens were separated into groups injected with either 3 or 7 mg/kg of BaP and euthanized after either 72 or 168 h. Then, we measured the thickness of the intestinal epithelium and the area occupied by MMCs. The thickness of intestinal epithelium decreased in both high and low concentration for short-term exposure compared to control, and increased in the long-term group in both low and high concentrations. The short-term decrease in thickness is due to the damage caused by BaP on the absorptive capacity of the epithelium, whereas the epithelium increased its thickness and recovered normal activity in the long-term. High BaP concentration decreased the area of MMCs in the short-term group. The increase in MMCs is associated with the detoxifying role of these cells, while the decrease was triggered by cellular stress due to high BaP concentration. The concentrations of BaP we used are close to those found in polluted environments. Therefore, water contaminated with BaP can potentially affect the morphology of internal organs of anurans. (AU)

• Processo FAPESP: 13/18469-5 - Morfologia do tubo digestório de Hypsiboas albopunctatus (Hylidae) e efeitos histológicos do contaminante benzo[alfa]pireno
• Beneficiário: Bruno Serra de Lacerda Valverde
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 13/02067-5 - Efeitos tóxicos dos herbicidas Atrazina e Roundup em Eupemphix nattereri (Leiuperidae) e Rhinella schneideri (Bufonidae): alterações morfológicas sob diferentes concentrações e condições abióticas (pH, temperatura e fotoperíodo)
• Beneficiário: Classius de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/05907-4 - Análises morfológicas do fígado, baço e testículos de Hypsiboas albopunctatus (Anura: Hylidae): efeitos do benzo[alfa]pireno
• Beneficiário: Lara Zácari Fanali
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 14/00946-4 - Pigmentação Visceral em Animais Ectotérmicos: Imunidade Inata e Defesa contra UV
• Beneficiário: Lilian Franco Belussi
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado