| Texto completo | |
| Autor(es): |
Borges, Felipe Azevedo
;
de Barros, Natan Roberto
;
Garms, Bruna Cambraia
;
Romeiro Miranda, Matheus Carlos
;
Padua Gemeinder, Jose Lucio
;
Ribeiro-Paes, Joao Tadeu
;
Silva, Rodrigo Ferreira
;
de Toledo, Karina Alves
;
Herculano, Rondinelli Donizetti
Número total de Autores: 9
|
| Tipo de documento: | Artigo Científico |
| Fonte: | Journal of Applied Polymer Science; v. 134, n. 39 OCT 15 2017. |
| Citações Web of Science: | 11 |
| Resumo | |
Natural rubber latex (NRL) from Hevea brasiliensis is a colloidal system composed of cis-1,4-polyisoprene. Its applications have grown due its angiogenic and wound healing activity. NRL has been used in guided bone regeneration as barrier, enhancing bone formation. However, there has been no study reported so far which shows its in vitro biocompatibility with osteoblasts. Thus, the aim of this work was to apply thermally induced phase separation under several temperatures to induce porosity in NRL; and test its mechanical properties, cytotoxicity, cell adhesion, and mineralization with MC3T3-E1. Only biomembranes submitted at -20 and -10 degrees C presented porosity. Fourier transform infrared spectroscopy showed no change in cis-1,4-isoprene spectra. Biomembranes were elastic (Young's modulus <1 MPa). According to ISO10993-5, NRL showed no cytotoxicity. Cells adhered on the NRL and produced mineral matrix as analyzed by scanning electron microscopy-energy-dispersive spectrometry, von kossa, and Fourier transform infrared spectroscopy. Cells on NRL presented higher alkaline phosphatase activity, however, mineralization showed no difference by alizarin red S dye extraction. (c) 2017 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2017, 134, 45321. (AU) | |
| Processo FAPESP: | 14/17526-8 - Desenvolvimento de um novo sistema empregando o látex natural como liberador de extratos vegetais reforçados por nanopartículas: caracterização física, química e biológica |
| Beneficiário: | Rondinelli Donizetti Herculano |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 11/17411-8 - Avaliação da liberação controlada de fármacos utilizando membranas de látex natural como carreador |
| Beneficiário: | Rondinelli Donizetti Herculano |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |