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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Application of natural rubber latex as scaffold for osteoblast to guided bone regeneration

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Author(s):
Borges, Felipe Azevedo ; de Barros, Natan Roberto ; Garms, Bruna Cambraia ; Romeiro Miranda, Matheus Carlos ; Padua Gemeinder, Jose Lucio ; Ribeiro-Paes, Joao Tadeu ; Silva, Rodrigo Ferreira ; de Toledo, Karina Alves ; Herculano, Rondinelli Donizetti
Total Authors: 9
Document type: Journal article
Source: Journal of Applied Polymer Science; v. 134, n. 39 OCT 15 2017.
Web of Science Citations: 11
Abstract

Natural rubber latex (NRL) from Hevea brasiliensis is a colloidal system composed of cis-1,4-polyisoprene. Its applications have grown due its angiogenic and wound healing activity. NRL has been used in guided bone regeneration as barrier, enhancing bone formation. However, there has been no study reported so far which shows its in vitro biocompatibility with osteoblasts. Thus, the aim of this work was to apply thermally induced phase separation under several temperatures to induce porosity in NRL; and test its mechanical properties, cytotoxicity, cell adhesion, and mineralization with MC3T3-E1. Only biomembranes submitted at -20 and -10 degrees C presented porosity. Fourier transform infrared spectroscopy showed no change in cis-1,4-isoprene spectra. Biomembranes were elastic (Young's modulus <1 MPa). According to ISO10993-5, NRL showed no cytotoxicity. Cells adhered on the NRL and produced mineral matrix as analyzed by scanning electron microscopy-energy-dispersive spectrometry, von kossa, and Fourier transform infrared spectroscopy. Cells on NRL presented higher alkaline phosphatase activity, however, mineralization showed no difference by alizarin red S dye extraction. (c) 2017 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2017, 134, 45321. (AU)

FAPESP's process: 14/17526-8 - Development of a novel system employing natural rubber latex as releasing of vegetable extracts incorporated with nanoparticles: physical chemical and biological characterization
Grantee:Rondinelli Donizetti Herculano
Support Opportunities: Regular Research Grants
FAPESP's process: 11/17411-8 - Evaluation of drugs delivery systems using natural rubber latex membranes as carrier
Grantee:Rondinelli Donizetti Herculano
Support Opportunities: Regular Research Grants