Development of a novel system employing natural rubber latex as releasing of vegetable extracts incorporated with nanoparticles: physical chemical and biological characterization

• Grant number: 14/17526-8
• Support Opportunities: Regular Research Grants
• Start date: April 01, 2015
• End date: September 30, 2017
• Field of knowledge: Engineering - Biomedical Engineering - Medical Engineering
• Principal Investigator: Rondinelli Donizetti Herculano
• Grantee: Rondinelli Donizetti Herculano
• Host Institution: Faculdade de Ciências Farmacêuticas (FCFAR). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Associated researchers: Bruno de Camargo Drago ; Felipe Azevedo Borges ; Matheus Carlos Romeiro Miranda
• Associated research grant(s): 15/11759-3 - Multi-User Equipment approved in grant 2014/17526-8: electron paramagnetic resonance (EPR) spectrometer - X band, AP.EMU

Abstract

Natural Rubber Latex (NRL) has been proven excellent for its biocompatibility and ability to stimulate angiogenesis, cellular adhesion and the formation of extracellular matrix, promoting the replacement and regeneration of tissue. The aim of this project is to incorporate the extracts of Stryphnodendron sp, Casearia sylvestris Swartz and Cordia verbenacea in latex biomembranes and to study the extract delivery system behavior. Moreover, in this work these extracts will be loaded in metallic nanoparticles. Stryphnodendron sp, commonly known as barbatimão, is extensively used in folk medicine for the treatment of diarrhoea, gynaecological problems and for healing wounds. The Casearia sylvestris Sw., commonly known as "guaçatonga" and it is used by indigenous population from South America (Brazil, Peru and Bolivia) in the treatment of several diseases, including cancer. The Cordia Verbenacea DC. is an anti-inflammatory, anti-ulcer, anti-rheumatic and healing plant that has therapeutic properties and low toxicity, thanks to the action of the essential oil, which has the active ingredient alpha-humulene. Silver nanoparticles (AgNP), gold nanoparticles (AuNP) and magnetic nanoparticles (FeNP) are attracting attention due to their bactericidal properties, magnetic and carriers. Thus, in this work these extracts will be loaded in these metallic nanoparticles. For the extract release study, membranes with Stryphnodendron sp., C. sylvestris and C. verbenacea will be placed in 400 mL of SBF solution, where the release behavior will be observed. Aliquots of this solution will be collected during an interval ranging from 10 to 350 hours. The extracts release as a function of time will be determined using the UV-VIS method. The membranes with extracts load in nanoparticles will be characterized by XRD, FTIR, SEM and mechanical strength tests. In addition, cytotoxicity studies from fibroblast cells and pre-osteoblasts with samples will be analyzed. Finally, this project also will study the antioxidant capacity of extracts using the technique of electron paramagnetic resonance (EPR) and optical spectroscopy (UV-VIS) and quantification of phenols and total tannins of extracts (AU)

Scientific publications

(27)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DE BARROS, NATAN ROBERTO; ROMEIRO MIRANDA, MATHEUS CARLOS; BORGES, FELIPE AZEVEDO; DE MENDONCA, RICARDO JOSE; CILLI, EDUARDO MAFFUD; HERCULANO, RONDINELLI DONIZETTI. Oxytocin Sustained Release Using Natural Rubber Latex Membranes. INTERNATIONAL JOURNAL OF PEPTIDE RESEARCH AND THERAPEUTICS, v. 22, n. 4, p. 435-444, DEC 2016. (14/17526-8, 15/02343-8)

BORGES, FELIPE AZEVEDO; DE BARROS, NATAN ROBERTO; GARMS, BRUNA CAMBRAIA; ROMEIRO MIRANDA, MATHEUS CARLOS; PADUA GEMEINDER, JOSE LUCIO; RIBEIRO-PAES, JOAO TADEU; SILVA, RODRIGO FERREIRA; DE TOLEDO, KARINA ALVES; HERCULANO, RONDINELLI DONIZETTI. Application of natural rubber latex as scaffold for osteoblast to guided bone regeneration. Journal of Applied Polymer Science, v. 134, n. 39, OCT 15 2017. (14/17526-8, 11/17411-8)

ZANCANELA, DANIELA CERVELLE; HERCULANO, RONDINELLI DONIZETTI; FUNARI, CRISTIANO SOLEO; MARCOS, CAROLINE MARIA; FUSCO ALMEIDA, ANA MARISA; GUASTALDI, ANTONIO CARLOS. Physical, chemical and antimicrobial implications of the association of propolis with a natural rubber latex membrane. Materials Letters, v. 209, p. 39-42, DEC 15 2017. (14/17526-8)

GARMS, BRUNA CAMBRAIA; BORGES, FELIPE AZEVEDO; DE BARROS, NATAN ROBERTO; MARCELINO, MONICA YONASHIRO; LEITE, MARCEL NANI; DEL ARCO, MARINA CONSTANTE; DE SOUZA SALVADOR, SERGIO LUIZ; PEGORIN, GIOVANA SANT'ANA; MUSTAFE OLIVEIRA, KASSANDRA SUSSI; CIPRIANI FRADE, MARCO ANDREY; et al. Novel polymeric dressing to the treatment of infected chronic wound. Applied Microbiology and Biotechnology, v. 103, n. 12, p. 4767-4778, JUN 2019. (14/17526-8, 11/17411-8)

GARMS, B. C.; BORGES, F. A.; SANTOS, R. E.; NIGOGHOSSIAN, K.; MIRANDA, M. C. R.; MIRANDA, I. U.; DALTRO, P.; SCARPARI, S. L.; GIAGIO, R. J.; BARROS, N. R.; et al. Characterization and Microbiological Application of Ciprofloxacin Loaded in Natural Rubber Latex Membranes. BRITISH JOURNAL OF PHARMACEUTICAL RESEARCH, v. 15, n. 1, 2017. (14/17526-8, 11/17411-8)

TANAKA, JEAN LUCAS; COSTA-ORLANDI, CAROLINE BARCELOS; BURD, BETINA SAYEG; PEGORIN, GIOVANA SANT'ANA; DA SILVA, THAINA VENANCIO; GUERRA, NAYRIM BRIZUELA; SOARES MENDES-GIANNINI, MARIA JOSE; FUSCO-ALMEIDA, ANA MARISA; HERCULANO, RONDINELLI DONIZETTI; DE BARROS, NATAN ROBERTO. Natural rubber dressing loaded with silver sulfadiazine for the treatment of burn wounds infected with Candida spp.. International Journal of Biological Macromolecules, v. 189, p. 597-606, OCT 31 2021. (17/19603-8, 11/17411-8, 17/18388-6, 14/17526-8)

ZANCANELA, DANIELA CERVELLE; FUNARI, CRISTIANO SOLEO; HERCULANO, RONDINELLI DONIZETTI; MELLO, VINICIUS MOREIRA; RODRIGUES, CLENILSON MARTINS; BORGES, FELIPE AZEVEDO; DE BARROS, NATAN ROBERTO; MARCOS, CAROLINE MARIA; FUSCO ALMEIDA, ANA MARISA; GUASTALDI, ANTONIO CARLOS. Natural rubber latex membranes incorporated with three different types of propolis: Physical-chemistry and antimicrobial behaviours. Materials Science & Engineering C-Materials for Biological Applications, v. 97, p. 576-582, APR 2019. (15/11759-3, 17/19603-8, 14/17526-8, 16/17048-4)

LIMA, ALINE DE FREITAS; PEGORIN, GIOVANA SANT'ANA; ROMEIRO MIRANDA, MATHEUS CARLOS; CACHANESKI-LOPES, JOAO PAULO; SILVA, WILLIAM DE MELO; BORGES, FELIPE AZEVEDO; GUERRA, NAYRIM BRIZUELA; HERCULANO, RONDINELLI DONIZETTI; BATAGIN-NETO, AUGUSTO. Ibuprofen-loaded biocompatible latex membrane for drug release: Characterization and molecular modeling. JOURNAL OF APPLIED BIOMATERIALS & FUNCTIONAL MATERIALS, v. 19, MAR 2021. (11/17411-8, 14/17526-8, 17/19603-8)

ROMEIRO MIRANDA, MATHEUS CARLOS; SATO, NICOLA CARLUCCI; PEGORIN BRASIL, GIOVANA SANT'ANA; PIAZZA, RODOLFO DEBONE; JAFELICCI JR, MIGUEL; DE BARROS, NATAN ROBERTO; BORGES, FELIPE AZEVEDO; BATAGIN-NETO, AUGUSTO; SILVA, WILLIAM DE MELO; HERCULANO, RONDINELLI DONIZETTI; et al. Silver nanoparticles effect on drug release of metronidazole in natural rubber latex dressing. POLYMER BULLETIN, DEC 2021. (14/17526-8, 11/17411-8)

ROMEIRA, KAROLINE MANSANO; ABDALLA, GABRIELA; GONCALVES, ROGERIO PENNA; PEGORIN, GIOVANA SANT'ANA; DE AZEREDO, HENRIETTE MONTEIRO CORDEIRO; MUSSAGY, CASSAMO USSEMANE; HERCULANO, RONDINELLI DONIZETTI. Residual Starch Packaging Derived from Potato Washing Slurries to Preserve Fruits. Food and Bioprocess Technology, v. 14, n. 12, OCT 2021. (11/17411-8, 17/19603-8, 14/17526-8)

ROMEIRO MIRANDA, MATHEUS CARLOS; BORGES, FELIPE AZEVEDO; BARROS, NATAN ROBERTO; MARQUES, MARINA PAGANINE; GALEANE, MARIANA CRISTINA; SINGULANI, JUNYA DE LACORTE; GUERRA, NAYRIM BRIZUELA; PEGORIN BRASIL, GIOVANA SANT'ANA; MUSSAGY, CASSAMO USSEMANE; FUSCO ALMEIDA, ANA MARISA; et al. In vitro and alternative animal models to evaluate the biocompatibility of natural latex-calcium phosphate-based polymer. JOURNAL OF POLYMERS AND THE ENVIRONMENT, v. N/A, p. 11-pg., 2022-04-05. (14/17526-8, 17/19603-8)

BORGES, FELIPE AZEVEDO; DRAGO, BRUNO DE CAMARGO; BAGGIO, LUIS OTAVIO; DE BARROS, NATAN ROBERTO; PEGORIN BRASIL, GIOVANA SANT'ANA; SCONTRI, MATEUS; MUSSAGY, CASSAMO USSEMANE; DA SILVA RIBEIRO, MATHEUS CICERO; PEREIRA MILORI, DEBORA MARCONDES BASTOS; DE MORAIS, CARLA PEREIRA; et al. Metronidazole-loaded gold nanoparticles in natural rubber latex as a potential wound dressing. International Journal of Biological Macromolecules, v. 211, p. 12-pg., 2022-05-18. (12/12958-1, 14/17526-8, 11/17411-8, 20/08655-0)

BURD, BETINA SAYEG; USSEMANE MUSSAGY, CASSAMO; SINGULANI, JUNYA DE LACORTE; TANAKA, JEAN LUCAS; SCONTRI, MATEUS; PEGORIN BRASIL, GIOVANA SANT'ANA; GUERRA, NAYRIM BRIZUELA; ASSATO, PATRICIA AKEMI; DE SOUSA ABREU, ANA PAULA; BEBBER, CAMILA CALDERAN; et al. Galleria Mellonella Larvae as an Alternative to Low-Density Polyethylene and Polystyrene Biodegradation. JOURNAL OF POLYMERS AND THE ENVIRONMENT, v. 31, n. 3, p. 10-pg., 2022-11-24. (14/17526-8, 11/17411-8, 17/19603-8)

FLORIANO, JULIANA F.; CHAO, VITORIA DE SOUSA; BOLOGNESI, LUIS F. C.; DE BARROS, NATAN R.; MIRANDA, MATHEUS C. R.; BORGES, FELIPE A.; CHAGAS, ANA LAURA D.; PAULINO, CRISTIANE G.; GARMS, BRUNA C.; MARCELINO, MONICA Y.; et al. Physical, Chemical and Biological Characterization of Natural Rubber Latex Membranes Loaded with Cordia verbenacea DC. Extract. CURRENT TRADITIONAL MEDICINE, v. 4, n. 2, p. 15-pg., 2018-01-01. (11/17411-8, 14/17526-8, 14/11037-5)

FLORIANO, JULIANA F.; CHAO, VITORIA DE SOUSA; BOLOGNESI, LUIS F. C.; DE BARROS, NATAN R.; MIRANDA, MATHEUS C. R.; BORGES, FELIPE A.; CHAGAS, ANA LAURA D.; PAULINO, CRISTIANE G.; GARMS, BRUNA C.; MARCELINO, MONICA Y.; et al. Physical, Chemical and Biological Characterization of Natural Rubber Latex Membranes Loaded with Cordia verbenacea DC. Extract. JOURNAL OF FUNGI, v. 4, n. 2, p. 140-154, 2018. (14/17526-8, 14/11037-5, 11/17411-8)

MORISE, B. T.; CHAGAS, A. L. D.; BARROS, N. R.; MIRANDA, M. C. R.; BORGES, F. A.; GEMEINDER, J. L. P.; SILVA, R. G.; PAULINO, C. G.; HERCULANO, R. D.; NORBERTO, A. M. Q.. Scopolamine loaded in natural rubber latex as a future transdermal patch for sialorrhea treatment. INTERNATIONAL JOURNAL OF POLYMERIC MATERIALS, v. 68, n. 13, p. 788-795, SEP 2 2019. (14/17526-8, 11/17411-8)

DE BARROS, NATAN ROBERTO; DOS SANTOS, RICARDO SOARES; ROMEIRO MIRANDA, MATHEUS CARLOS; CESAR BOLOGNESI, LUIS FELIPE; BORGES, FELIPE AZEVEDO; SCHIAVON, JOAO VICTOR; COSTA MARQUES, RODRIGO FERNANDO; HERCULANO, RONDINELLI DONIZETTI; QUEIROS NORBERTO, ANA MARIA. Natural latex-glycerol dressing to reduce nipple pain and healing the skin in breastfeeding women. SKIN RESEARCH AND TECHNOLOGY, v. 25, n. 4, p. 461-468, JUL 2019. (14/17526-8, 17/19603-8)

PEGORIN BRASIL, GIOVANA SANT'ANA; DE BARROS, PATRICIA PIMENTEL; MIRANDA, MATHEUS CARLOS ROMEIRO; DE BARROS, NATAN ROBERTO; JUNQUEIRA, JULIANA CAMPOS; GOMEZ, ALEJANDRO; HERCULANO, RONDINELLI DONIZETTI; DE MENDONCA, RICARDO JOSE. Natural latex serum: characterization and biocompatibility assessment using Galleria mellonella as an alternative in vivo model. JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION, DEC 2021. (14/17526-8, 11/17411-8)

MARCATTO, VINICIUS ASSIS; PEGORIN, GIOVANA SANT'ANA; BARBOSA, GUSTAVO FRANCO; HERCULANO, RONDINELLI DONIZETTI; GUERRA, NAYRIM BRIZUELA. 3D printed-polylactic acid scaffolds coated with natural rubber latex for biomedical application. Journal of Applied Polymer Science, v. 139, n. 9, OCT 2021. (14/17526-8, 17/19603-8, 11/17411-8)

DE PAIVA, MARCELA BRITTO; BRASIL, GIOVANA SANT'ANA PEGORIN; CHAGAS, ANA LAURA DESTRO; MACEDO, ANA PAULA; RAMOS, JUNIA; ISSA, JOAO PAULO MARDEGAN; GANGRADE, ANKIT; FLORIANO, JULIANA FERREIRA; CAETANO, GUILHERME FERREIRA; LI, BINGBING; et al. Latex-collagen membrane: an alternative treatment for tibial bone defects. Journal of Materials Science, v. N/A, p. 23-pg., 2022-12-08. (14/17526-8, 11/17411-8, 17/19603-8, 17/20051-0)

MARCELINO, MONICA YONASHIRO; BORGES, FELIPE AZEVEDO; MARTINS COSTA, ANA FLAVIA; SINGULANI, JUNYA DE LACORTE; RIBEIRO, NATHANVINICIUS; COSTA-ORLANDI, CAROLINE BARCELOS; GARMS, BRUNA CAMBRAIA; MENDES-GIANNINI, MARIA JOSE SOARES; HERCULANO, RONDINELLI DONIZETTI; FUSCO-ALMEIDA, ANA MARISA. Antifungal activity of fluconazole-loaded natural rubber latex against Candida albicans. FUTURE MICROBIOLOGY, v. 13, n. 3, p. 359-367, MAR 2018. (14/17526-8, 11/17411-8)

FLORIANO, JULIANA FERREIRA; DE BARROS, NATAN ROBERTO; FERREIRA CINMAN, JOSE LUIZ; DA SILVA, ROSANGELA GONCALVES; LOFFREDO, AUGUSTO VILLELA; BORGES, FELIPE AZEVEDO; QUEIROS NORBERTO, ANA MARIA; DESTRO CHAGAS, ANA LAURA; GARMS, BRUNA CAMBRAIA; DE OLIVEIRA GRAEFF, CARLOS FREDERICO; et al. Ketoprofen Loaded in Natural Rubber Latex Transdermal Patch for Tendinitis Treatment. JOURNAL OF POLYMERS AND THE ENVIRONMENT, v. 26, n. 6, p. 2281-2289, JUN 2018. (14/17526-8, 11/17411-8)

BORGES, FELIPE AZEVEDO; DE ALMEIDA FILHO, EDSON; ROMEIRO MIRANDA, MATHEUS CARLOS; DOS SANTOS, MARCIO LUIZ; HERCULANO, RONDINELLI DONIZETTI; GUASTALDI, ANTONIO CARLOS. Natural rubber latex coated with calcium phosphate for biomedical application. JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION, v. 26, n. 17, p. 1256-1268, NOV 22 2015. (14/17526-8, 11/17411-8)

DE BARROS, NATAN ROBERTO; HEREDIA-VIEIRA, SILVIA CRISTINA; BORGES, FELIPE AZEVEDO; BENITES, NIKOLLAS MOREIRA; DOS REIS, CAMILA EUGENIA; ROMEIRO MIRANDA, MATHEUS CARLOS; LIMA CARDOSO, CLAUDIA ANDREA; HERCULANO, RONDINELLI DONIZETTI. Natural rubber latex biodevice as controlled release system for chronic wounds healing. BIOMEDICAL PHYSICS & ENGINEERING EXPRESS, v. 4, n. 3, MAY 2018. (15/11759-3, 14/17526-8)

MUSSAGY, CASSAMO U.; OSHIRO, ARIANE A.; LIMA, CAIO A.; AMANTINO, CAMILA F.; PRIMO, FERNANDO L.; SANTOS-EBINUMA, VALERIA C.; HERCULANO, RONDINELLI D.. Natural fluorescent red colorants produced by Talaromyces amestolkiae as promising coloring agents for custom-made latex gloves. JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY, v. 119, p. 10-pg., 2023-01-23. (11/17411-8, 20/08655-0, 19/15493-9, 21/06686-8, 14/17526-8)

PADUA GEMEINDER, JOSE LUCIO; DE BARROS, NATAN ROBERTO; PEGORIN, GIOVANA SANT'ANA; SINGULANI, JUNYA DE LACORTE; BORGES, FELIPE AZEVEDO; GABRIEL DEL ARCO, MARINA CONSTANTE; SOARES MENDES GIANNINI, MARIA JOSE; FUSCO ALMEIDA, ANA MARISA; DE SOUZA SALVADOR, SERGIO LUIZ; HERCULANO, RONDINELLI DONIZETTI. Gentamicin encapsulated within a biopolymer for the treatment ofStaphylococcus aureusandEscherichia coliinfected skin ulcers. JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION, v. 32, n. 1, SEP 2020. (11/17411-8, 14/17526-8)

MARIA CANAAN, JOSIANE MARCIA; PEGORIN BRASIL, GIOVANA SANT'ANA; DE BARROS, NATAN ROBERTO; MUSSAGY, CASSAMO USSEMANE; GUERRA, NAYRIM BRIZUELA; HERCULANO, RONDINELLI DONIZETTI. Soybean processing wastes and their potential in the generation of high value added products. Food Chemistry, v. 373, n. B, MAR 30 2022. (17/19603-8, 20/08655-0, 14/17526-8)