Development of a novel patch containing latex and barbatimam e fractions and study of signaling pathways in cutaneous wound repair

• Grant number: 17/19603-8
• Support Opportunities: Regular Research Grants
• Duration: May 01, 2018 - December 31, 2020
• Field of knowledge: Engineering - Biomedical Engineering - Bioengineering
• Principal Investigator: Rondinelli Donizetti Herculano
• Grantee: Rondinelli Donizetti Herculano
• Host Institution: Faculdade de Ciências Farmacêuticas (FCFAR). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Associated researchers: Felipe Azevedo Borges ; Natan Roberto de Barros

Abstract

Natural rubber latex membranes of Hevea brasiliensis has been used as a dressing for cutaneous ulcers. Moreover, latex membrane also has been broadly used in controlled drug release systems development. Barbatimam have several medically useful properties, such as anti-inflammatory, antimicrobial, antioxidant and healing properties, associated to high tannin contents. Its organic fraction (ethyl acetate) presents ~ 20% total tannin content in the composition. The aim of this work is to evaluate the barbatimam extract and fractions release by latex membrane employing in a High Performance Liquid Chromatography (HPLC). Evaluation of surface roughness and morphology of different porous membrane will realized by Atomic Force Microscopy (AFM). It will also be analyzed the adhesion of fibroblasts cells on porous membranes by AFM. Peptide Mass Fingerprinting (PMF) analyzes of fibroblast cultures will be performed to detect differentially expressed proteins as a result of the action of barbatimao extract, fractions, latex membrane and natural rubber latex membrane with compounds exposed to the cells. After that, molecular signaling analysis will be performed using real time PCR (qPCR) and Western blotting techniques in these cultures. In this way, it is hoped that the results can indicates this product as a new technology in favor of the human benefit. (AU)

Scientific publications

(24)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PEGORIN, GIOVANA S.; LEITE, MARCEL N.; ANTONIASSI, MARCIO; CHAGAS, ANA LAURA D.; SANTANA, LUISIANE A.; GARMS, BRUNA C.; MARCELINO, MONICA Y.; HERCULANO, RONDINELLI D.; CIPRIANI FRADE, MARCO ANDREY. Physico-chemical characterization and tissue healing changes by Hancornia speciosa Gomes latex biomembrane. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, v. 109, n. 7, NOV 2020. (17/19603-8, 09/09355-0, 16/16437-7)

ZANCANELA, DANIELA CERVELLE; FUNARI, CRISTIANO SOLEO; HERCULANO, RONDINELLI DONIZETTI; MELLO, VINICIUS MOREIRA; RODRIGUES, CLENILSON MARTINS; BORGES, FELIPE AZEVEDO; DE BARROS, NATAN ROBERTO; MARCOS, CAROLINE MARIA; FUSCO ALMEIDA, ANA MARISA; GUASTALDI, ANTONIO CARLOS. Natural rubber latex membranes incorporated with three different types of propolis: Physical-chemistry and antimicrobial behaviours. Materials Science & Engineering C-Materials for Biological Applications, v. 97, p. 576-582, APR 2019. (15/11759-3, 17/19603-8, 14/17526-8, 16/17048-4)

DA SILVA, V, THAINA; DE BARROS, NATAN R.; COSTA-ORLANDI, CAROLINE B.; TANAKA, JEAN L.; MORO, LINCOLN G.; PEGORIN, GIOVANA S.; OLIVEIRA, KASSANDRA S. M.; MENDES-GIANINNI, MARIA J. S.; FUSCO-ALMEIDA, ARIA M.; HERCULANO, RONDINELLI D.. Voriconazole-natural latex dressings for treating infected Candida spp. skin ulcers. FUTURE MICROBIOLOGY, v. 15, n. 15, p. 1439-1452, OCT 2020. (17/19603-8, 17/18388-6)

BARROS, NATAN ROBERTO; AHADIAN, SAMAD; TEBON, PEYTON; CUNHA RUDGE, MARILZA VIEIRA; PASCON BARBOSA, ANGELICA MERCIA; HERCULANO, RONDINELLI DONIZETTI. Highly absorptive dressing composed of natural latex loaded with alginate for exudate control and healing of diabetic wounds. Materials Science & Engineering C-Materials for Biological Applications, v. 119, FEB 2021. (17/19603-8, 17/02518-8, 16/01743-5)

SPIN, JOSE RODOLFO; DE OLIVEIRA, GUILHERME JOSE PIMENTEL LOPES; SPIN-NETO, RUBENS; HERCULANO, RONDINELLI DONIZETTI; MARCANTONIO, ROSEMARY ADRIANA CHIERICI. Effect of natural latex membranes on wound repair of palate donor areas: A pilot randomized controlled trial study, including the membranes characterization. MATERIALS TODAY COMMUNICATIONS, v. 27, JUN 2021. (17/19603-8, 16/00275-8)

TANAKA, JEAN LUCAS; COSTA-ORLANDI, CAROLINE BARCELOS; BURD, BETINA SAYEG; PEGORIN, GIOVANA SANT'ANA; DA SILVA, THAINA VENANCIO; GUERRA, NAYRIM BRIZUELA; SOARES MENDES-GIANNINI, MARIA JOSE; FUSCO-ALMEIDA, ANA MARISA; HERCULANO, RONDINELLI DONIZETTI; DE BARROS, NATAN ROBERTO. Natural rubber dressing loaded with silver sulfadiazine for the treatment of burn wounds infected with Candida spp.. International Journal of Biological Macromolecules, v. 189, p. 597-606, OCT 31 2021. (17/19603-8, 11/17411-8, 17/18388-6, 14/17526-8)

LIMA, ALINE DE FREITAS; PEGORIN, GIOVANA SANT'ANA; ROMEIRO MIRANDA, MATHEUS CARLOS; CACHANESKI-LOPES, JOAO PAULO; SILVA, WILLIAM DE MELO; BORGES, FELIPE AZEVEDO; GUERRA, NAYRIM BRIZUELA; HERCULANO, RONDINELLI DONIZETTI; BATAGIN-NETO, AUGUSTO. Ibuprofen-loaded biocompatible latex membrane for drug release: Characterization and molecular modeling. JOURNAL OF APPLIED BIOMATERIALS & FUNCTIONAL MATERIALS, v. 19, MAR 2021. (11/17411-8, 14/17526-8, 17/19603-8)

ROMEIRO MIRANDA, MATHEUS CARLOS; BORGES, FELIPE AZEVEDO; BARROS, NATAN ROBERTO; MARQUES, MARINA PAGANINE; GALEANE, MARIANA CRISTINA; SINGULANI, JUNYA DE LACORTE; GUERRA, NAYRIM BRIZUELA; PEGORIN BRASIL, GIOVANA SANT'ANA; MUSSAGY, CASSAMO USSEMANE; FUSCO ALMEIDA, ANA MARISA; et al. In vitro and alternative animal models to evaluate the biocompatibility of natural latex-calcium phosphate-based polymer. JOURNAL OF POLYMERS AND THE ENVIRONMENT, v. N/A, p. 11-pg., 2022-04-05. (14/17526-8, 17/19603-8)

HERCULANO, RONDINELLI DONIZETTI; DOS SANTOS, THAINA OLIVEIRA; DE BARROS, NATAN ROBERTO; PEGORIN BRASIL, GIOVANA SANT'ANA; SCONTRI, MATEUS; CARVALHO, BRUNA GREGATTI; MECWAN, MARVIN; FARHADI, NEDA; KAWAKITA, SATORU; PEREGO, CAIO HUMBERTO; et al. Aloe vera-loaded natural rubber latex dressing as a potential complementary treatment for psoriasis. International Journal of Biological Macromolecules, v. 242, p. 13-pg., 2023-05-12. (18/07342-8, 17/19603-8, 21/09207-3)

SILVA, FLAVIO KUNERT DE SOUZA; COSTA-ORLANDI, CAROLINE BARCELOS; FERNANDES, MARIZA AIRES; BRASIL, GIOVANA SANT'ANA PEGORIN; MUSSAGY, CASSAMO USSEMANE; SCONTRI, MATEUS; SASAKI, JOSANA CARLA DA SILVA; ABREU, ANA PAULA DE SOUSA; GUERRA, NAYRIM BRIZUELA; FLORIANO, JULIANA FERREIRA; et al. Biocompatible anti-aging face mask prepared with curcumin and natural rubber with antioxidant properties. International Journal of Biological Macromolecules, v. 242, p. 12-pg., 2023-05-13. (18/07342-8, 17/19603-8, 21/09207-3)

ROMEIRA, KAROLINE MANSANO; ABDALLA, GABRIELA; GONCALVES, ROGERIO PENNA; PEGORIN, GIOVANA SANT'ANA; DE AZEREDO, HENRIETTE MONTEIRO CORDEIRO; MUSSAGY, CASSAMO USSEMANE; HERCULANO, RONDINELLI DONIZETTI. Residual Starch Packaging Derived from Potato Washing Slurries to Preserve Fruits. Food and Bioprocess Technology, v. 14, n. 12, OCT 2021. (11/17411-8, 17/19603-8, 14/17526-8)

ABDALLA, GABRIELA; MUSSAGY, CASSAMO USSEMANE; BRASIL, GIOVANA SANT'ANA PEGORIN; SCONTRI, MATEUS; SASAKI, JOSANA CARLA DA SILVA; SU, YANJIN; BEBBER, CAMILA; ROCHA, RAILDIS RIBEIRO; ABREU, ANA PAULA DE SOUSA; GONCALVES, ROGERIO PENNA; et al. Eco-sustainable coatings based on chitosan, pectin, and lemon essential oil nanoemulsion and their effect on strawberry preservation. International Journal of Biological Macromolecules, v. 249, p. 15-pg., 2023-09-30. (18/07342-8, 18/21887-7, 17/19603-8, 11/17411-8, 15/11759-3)

BURD, BETINA SAYEG; USSEMANE MUSSAGY, CASSAMO; SINGULANI, JUNYA DE LACORTE; TANAKA, JEAN LUCAS; SCONTRI, MATEUS; PEGORIN BRASIL, GIOVANA SANT'ANA; GUERRA, NAYRIM BRIZUELA; ASSATO, PATRICIA AKEMI; DE SOUSA ABREU, ANA PAULA; BEBBER, CAMILA CALDERAN; et al. Galleria Mellonella Larvae as an Alternative to Low-Density Polyethylene and Polystyrene Biodegradation. JOURNAL OF POLYMERS AND THE ENVIRONMENT, v. 31, n. 3, p. 10-pg., 2022-11-24. (14/17526-8, 11/17411-8, 17/19603-8)

CESAR, MARIANA BIONDI; BORGES, FELIPE AZEVEDO; BILCK, ANA PAULA; YAMASHITA, FABIO; PAULINO, CRISTIANE GARCIA; HERCULANO, RONDINELLI DONIZETTI. Development and Characterization of Natural Rubber Latex and Polylactic Acid Membranes for Biomedical Application. JOURNAL OF POLYMERS AND THE ENVIRONMENT, OCT 2019. (16/09736-8, 17/19603-8)

DE BARROS, NATAN ROBERTO; DOS SANTOS, RICARDO SOARES; ROMEIRO MIRANDA, MATHEUS CARLOS; CESAR BOLOGNESI, LUIS FELIPE; BORGES, FELIPE AZEVEDO; SCHIAVON, JOAO VICTOR; COSTA MARQUES, RODRIGO FERNANDO; HERCULANO, RONDINELLI DONIZETTI; QUEIROS NORBERTO, ANA MARIA. Natural latex-glycerol dressing to reduce nipple pain and healing the skin in breastfeeding women. SKIN RESEARCH AND TECHNOLOGY, v. 25, n. 4, p. 461-468, JUL 2019. (14/17526-8, 17/19603-8)

CESAR, MARIANA BIONDI; BORGES, FELIPE AZEVEDO; BILCK, ANA PAULA; YAMASHITA, FABIO; PAULINO, CRISTIANE GARCIA; HERCULANO, RONDINELLI DONIZETTI. Development and Characterization of Natural Rubber Latex and Polylactic Acid Membranes for Biomedical Application. JOURNAL OF POLYMERS AND THE ENVIRONMENT, v. 28, n. 1, p. 11-pg., 2019-10-31. (16/09736-8, 17/19603-8)

MARCATTO, VINICIUS ASSIS; PEGORIN, GIOVANA SANT'ANA; BARBOSA, GUSTAVO FRANCO; HERCULANO, RONDINELLI DONIZETTI; GUERRA, NAYRIM BRIZUELA. 3D printed-polylactic acid scaffolds coated with natural rubber latex for biomedical application. Journal of Applied Polymer Science, v. 139, n. 9, OCT 2021. (14/17526-8, 17/19603-8, 11/17411-8)

CESAR, MARIANA BIONDI; POLI, HAMISH; PIAZZA, RODOLFO DEBONE; COSTA MARQUES, RODRIGO FERNANDO; HERCULANO, RONDINELLI DONIZETTI; GRONDAHL, LISBETH. Dispersion of hydroxyapatite nanoparticles in natural rubber latex and poly lactic acid based matrices. Journal of Applied Polymer Science, v. 139, n. 20, p. 15-pg., 2022-01-28. (17/19603-8, 16/09736-8, 17/16324-0)

DE PAIVA, MARCELA BRITTO; BRASIL, GIOVANA SANT'ANA PEGORIN; CHAGAS, ANA LAURA DESTRO; MACEDO, ANA PAULA; RAMOS, JUNIA; ISSA, JOAO PAULO MARDEGAN; GANGRADE, ANKIT; FLORIANO, JULIANA FERREIRA; CAETANO, GUILHERME FERREIRA; LI, BINGBING; et al. Latex-collagen membrane: an alternative treatment for tibial bone defects. Journal of Materials Science, v. N/A, p. 23-pg., 2022-12-08. (14/17526-8, 11/17411-8, 17/19603-8, 17/20051-0)

NICOLODELLI, GUSTAVO; HERCULANO, RONDINELLI D.; MARANGONI, BRUNO S.; RIBEIRO, MATHEUS C. S.; MILORI, DEBORA M. B. P.; MENEGATTI, CARLOS R.. Differentiation of latex biomembrane with collagen and non-collagen using laser induced breakdown spectroscopy. MATERIALS TODAY COMMUNICATIONS, v. 30, MAR 2022. (13/07276-1, 17/19603-8, 17/19248-3)

MARCELINO, MONICA YONASHIRO; BORGES, FELIPE AZEVEDO; SCORZONI, LILIANA; SINGULANI, JUNYA DE LACORTE; GARMS, BRUNA CAMBRAIA; NIEMEYER, JULIA CARINA; GUERRA, NAYRIM BRIZUELA; PEGORIN BRASIL, GIOVANA SANT'ANA; MUSSAGY, CASSAMO USSEMANE; DE OLIVEIRA CARVALHO, FRANCISCO ADRIANO; et al. Synthesis and characterization of gold nanoparticles and their toxicity in alternative methods to the use of mammals. JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING, v. 9, n. 6, DEC 2021. (17/19603-8, 16/11836-0)

GUERRA, NAYRIM BRIZUELA; PEGORIN, GIOVANA SANT'ANA; BORATTO, MIGUEL HENRIQUE; DE BARROS, NATAN ROBERTO; DE OLIVEIRA GRAEFF, CARLOS FREDERICO; HERCULANO, RONDINELLI DONIZETTI. Biomedical applications of natural rubber latex from the rubber tree Hevea brasiliensis. Materials Science & Engineering C-Materials for Biological Applications, v. 126, JUL 2021. (17/19603-8)

MARIA CANAAN, JOSIANE MARCIA; PEGORIN BRASIL, GIOVANA SANT'ANA; DE BARROS, NATAN ROBERTO; MUSSAGY, CASSAMO USSEMANE; GUERRA, NAYRIM BRIZUELA; HERCULANO, RONDINELLI DONIZETTI. Soybean processing wastes and their potential in the generation of high value added products. Food Chemistry, v. 373, n. B, MAR 30 2022. (17/19603-8, 20/08655-0, 14/17526-8)

PEGORIN, GIOVANA S.; LEITE, MARCEL N.; ANTONIASSI, MARCIO; CHAGAS, ANA LAURA D.; SANTANA, LUISIANE A.; GARMS, BRUNA C.; MARCELINO, MONICA Y.; HERCULANO, RONDINELLI D.; CIPRIANI FRADE, MARCO ANDREY. Physico-chemical characterization and tissue healing changes by Hancornia speciosa Gomes latex biomembrane. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, v. 109, n. 7, p. 11-pg., 2020-11-25. (09/09355-0, 16/16437-7, 17/19603-8)