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Development of a novel patch containing latex and barbatimam e fractions and study of signaling pathways in cutaneous wound repair

Grant number: 17/19603-8
Support type:Regular Research Grants
Duration: May 01, 2018 - October 31, 2020
Field of knowledge:Engineering - Biomedical Engineering - Bioengineering
Principal Investigator:Rondinelli Donizetti Herculano
Grantee:Rondinelli Donizetti Herculano
Home Institution: Faculdade de Ciências Farmacêuticas (FCFAR). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
Assoc. researchers:Felipe Azevedo Borges ; Natan Roberto de Barros

Abstract

Natural rubber latex membranes of Hevea brasiliensis has been used as a dressing for cutaneous ulcers. Moreover, latex membrane also has been broadly used in controlled drug release systems development. Barbatimam have several medically useful properties, such as anti-inflammatory, antimicrobial, antioxidant and healing properties, associated to high tannin contents. Its organic fraction (ethyl acetate) presents ~ 20% total tannin content in the composition. The aim of this work is to evaluate the barbatimam extract and fractions release by latex membrane employing in a High Performance Liquid Chromatography (HPLC). Evaluation of surface roughness and morphology of different porous membrane will realized by Atomic Force Microscopy (AFM). It will also be analyzed the adhesion of fibroblasts cells on porous membranes by AFM. Peptide Mass Fingerprinting (PMF) analyzes of fibroblast cultures will be performed to detect differentially expressed proteins as a result of the action of barbatimao extract, fractions, latex membrane and natural rubber latex membrane with compounds exposed to the cells. After that, molecular signaling analysis will be performed using real time PCR (qPCR) and Western blotting techniques in these cultures. In this way, it is hoped that the results can indicates this product as a new technology in favor of the human benefit. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
CESAR, MARIANA BIONDI; BORGES, FELIPE AZEVEDO; BILCK, ANA PAULA; YAMASHITA, FABIO; PAULINO, CRISTIANE GARCIA; HERCULANO, RONDINELLI DONIZETTI. Development and Characterization of Natural Rubber Latex and Polylactic Acid Membranes for Biomedical Application. JOURNAL OF POLYMERS AND THE ENVIRONMENT, OCT 2019. Web of Science Citations: 0.
DE BARROS, NATAN ROBERTO; DOS SANTOS, RICARDO SOARES; ROMEIRO MIRANDA, MATHEUS CARLOS; CESAR BOLOGNESI, LUIS FELIPE; BORGES, FELIPE AZEVEDO; SCHIAVON, JOAO VICTOR; COSTA MARQUES, RODRIGO FERNANDO; HERCULANO, RONDINELLI DONIZETTI; QUEIROS NORBERTO, ANA MARIA. Natural latex-glycerol dressing to reduce nipple pain and healing the skin in breastfeeding women. SKIN RESEARCH AND TECHNOLOGY, v. 25, n. 4, p. 461-468, JUL 2019. Web of Science Citations: 0.
ZANCANELA, DANIELA CERVELLE; FUNARI, CRISTIANO SOLEO; HERCULANO, RONDINELLI DONIZETTI; MELLO, VINICIUS MOREIRA; RODRIGUES, CLENILSON MARTINS; BORGES, FELIPE AZEVEDO; DE BARROS, NATAN ROBERTO; MARCOS, CAROLINE MARIA; FUSCO ALMEIDA, ANA MARISA; GUASTALDI, ANTONIO CARLOS. Natural rubber latex membranes incorporated with three different types of propolis: Physical-chemistry and antimicrobial behaviours. Materials Science & Engineering C-Materials for Biological Applications, v. 97, p. 576-582, APR 2019. Web of Science Citations: 3.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.