Development and physical, chemical and biological characterization of scopolamine loaded latex membrane for Sialorrhea treatment

• Grant number: 17/22686-2
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Effective date (Start): February 01, 2018
• Effective date (End): December 31, 2019
• Field of knowledge: Engineering - Biomedical Engineering - Bioengineering
• Principal Investigator: Rondinelli Donizetti Herculano
• Grantee: Beatriz Tiemi Morise
• Host Institution: Faculdade de Ciências Farmacêuticas (FCFAR). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Associated scholarship(s): 19/01650-5 - Development and characterization of scopolamine loaded in polycaprolactone matrices and natural rubber latex membranes modified by gamma-irradiation for sialorrhea treatment, BE.EP.IC

Abstract

The natural rubber latex (NRL) extracted from Hevea brasiliensis is widely used in industry and has shown good results in biomedical applications. Due its biocompatibility and ability to induce angiogenesis and bone regeneration, NRL can be used as medical prosthesis and grafts. Therefore NRL can be applied as a matrix of a controlled release system, allowing drugs to reach their target and prevent the occurrence of unwanted side effects. The confection of a controlled release system should be effective, in order to the drug release may occur gradually over a long period of time. Scopolamine is an anticholinergic agent commonly used for nausea and seasickness treatment. This drug can also be used for Sialorrhea treatment, a disorder which can promote an increased saliva production and usually occurs along with severe neurological disorders. The transdermal application of scopolamine minimizes the occurrence of side effect. Therefore, the objective of this project is produce NRL membranes carried with scopolamine in order to develop a possible transdermal patch, which can be used for Sialorrhea treatment. This project aims to analyze the release of the drug by NRL membranes using the UV-Vis spectroscopy technique and characterize theses membranes using Fourier transform infrared (FTIR) spectroscopy, high resolution scanning electron microscopy (SEM-FEG), tensile tests and contact angle analysis. In addition, the biological evaluation of the released drug and NRL membranes will be performed through analysis of the hemolytic activity, using commercial sheep blood, and tests of cell viability in fibroblast cultures (3T3 cells). (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MORISE, BEATRIZ TIEMI; MUTCH, ALEXANDRA LOUISE; GARMS, BRUNA CAMBRAIA; HERCULANO, RONDINELLI DONIZETTI; GRONDAHL, LISBETH. Evaluation of acrylic acid grafting on the loading and release of scopolamine butylbromide from polymeric matrices for future sialorrhea treatment. Journal of Applied Polymer Science, v. 138, n. 13, NOV 2020. (17/22686-2, 19/01650-5)