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Production of 3D scaffolds containing different concentrations of graphene, hydroxyapatite and tricalcium phosphate surface coated with human bone morphogenetic and P-1 latex proteins

Grant number: 14/23662-1
Support type:Scholarships abroad - Research Internship - Doctorate
Effective date (Start): September 18, 2015
Effective date (End): September 17, 2016
Field of knowledge:Interdisciplinary Subjects
Principal Investigator:Marco Andrey Cipriani Frade
Grantee:Guilherme Ferreira Caetano
Supervisor abroad: Paulo Jorge da Silva Bártolo
Home Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
Local de pesquisa : University of Manchester, England  
Associated to the scholarship:13/09055-2 - Osteo-differentiation of human mesenchymal stem cells attached to natural latex proteins from the rubber tree Hevea brasiliensis on scaffold of polycaprolactone and polylactic acid (PCL/PLA) to bone xenografting, BP.DR


Bone defects assume importance in growing prevalence of chronic health conditions since fractures and critical defects should increase as the population ages. The bone healing depends on a dense vascular network that provides essential oxygen and nutrients, an important characteristic for the process of tissue regeneration. Conventional treatments require transplantation and extremely invasive approaches, therefore the challenge of developing new therapies and treatments is still growing. A promising proposal is to obtain tissue in laboratory using scaffolds for the growth and differentiation of mesenchymal stem cells and factors that enable a suitable neovascularization and osteoinduction for grafting. This project aims to investigate the effect of graphene on the mechanical and biological properties of a biopolymer-based scaffold (polycaprolactone) and the production of 3D bioactive scaffolds through the incorporation of hydroxyapatite (HA) and Tricalcium Phosphate (TCP) to increase the biological recognition and the recruitment of cells to colonize the scaffold hence enhancing the healing process of the bone. Furthermore, 3D bioactive scaffolds containing HA, TCP or Graphene will be surface coated with human bone morphogenetic proteins such as hBMP-2 and P-1 latex proteins from Hevea brasiliensis rubber tree, in order to improve bioactivity and increase cellular adhesion, proliferation and differentiation of human mesenchymal stem cells (hMSCs). The protein fraction F1 from latex extracted from Hevea brasiliensis rubber tree exhibit important angiogenic and healing activity, promising advantages for use in tissue engineering, coupled with the multipotentiality of hMSCs seeded onto the scaffolds. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
CAETANO, GUILHERME FERREIRA; WANG, WEIGUANG; CHIANG, WEI-HUNG; COOPER, GLEN; DIVER, CARL; BLAKER, JONNY JAMES; FRADE, MARCO ANDREY; BARTOLO, PAULO. 3D-Printed Poly(epsilon-caprolactone)/Graphene Scaffolds Activated with P1-Latex Protein for Bone Regeneration. 3D PRINTING AND ADDITIVE MANUFACTURING, v. 5, n. 2, p. 127-137, JUN 2018. Web of Science Citations: 7.
WANG, WEIGUANG; CAETANO, GUILHERME; AMBLER, WILLIAM STEPHEN; BLAKER, JONNY JAMES; FRADE, MARCO ANDREY; MANDAL, PARTHASARATHI; DIVER, CARL; BARTOLO, PAULO. Enhancing the Hydrophilicity and Cell Attachment of 3D Printed PCL/Graphene Scaffolds for Bone Tissue Engineering. MATERIALS, v. 9, n. 12 DEC 2016. Web of Science Citations: 46.

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