Development of ceramics scaffolds for application in tissue engineering

• Grant number: 22/10604-0
• Support Opportunities: Research Projects - Thematic Grants
• Start date: September 01, 2023
• End date: August 31, 2027
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Pathology
• Principal Investigator: Carlos Eduardo Ambrósio
• Grantee: Carlos Eduardo Ambrósio
• Host Institution: Faculdade de Zootecnia e Engenharia de Alimentos (FZEA). Universidade de São Paulo (USP). Pirassununga , SP, Brazil
• Pesquisadores principais: Eliria Maria de Jesus Agnolon Pallone ; Rosemary Aparecida de Carvalho
• Associated researchers: Cristiana Maria Pedroso Yoshida ; Daniele dos Santos Martins ; Fernanda Maria Vanin ; Jorge Lino Alves ; Jorge Vicente Lopes da Silva ; Juliana Kelmy Macário Barboza Daguano ; Kelly Cristine Santos Roballo ; Luiz Alberto Colnago ; Marcus Antônio Rossi Feliciano ; Maria Isabel Ribeiro Dias ; Silvio Henrique de Freitas ; Tiziana Angela Luisa Brevini ; Zilda de Castro Silveira
• Associated research grant(s): 24/02229-0 - Evaluation of in vitro osteogenic activity of alumina/zirconia bioceramic scaffolds covered with hyaluronic acid and hydroxyapatite, AP.R; 23/15459-0 - Multi-user equipment approved in grant 2022/10604-0: INVENIO-R infrared spectrometer, AP.EMU
• Associated scholarship(s): 25/04045-6 - Development of morphogenetic protein carrier systems for scaffold coating, BP.PD; 24/13703-4 - Al2O3/ZrO2 scaffolds with incorporation of antibiotic release systems, BP.PD; 24/14528-1 - Development of morphogenetic protein carrier systems for scaffold coating, BP.PD; + associated scholarships 24/17274-0 - Development of a alternative protocol for the treatment of equine cutaneous habronemosis, BP.IC; 24/14631-7 - Spectroscopic analyses in the mid- and near-infrared regions for the characterization of ceramic scaffolds applied as biomaterials, BP.TT; 24/06681-4 - Development of ceramic scaffolds for application in tissue engineering, BP.PD - associated scholarships

Abstract

Porous bioceramics called scaffolds have been studied for bone replacement and repair, with properties that meet the functionality and integration between the biomaterial and the biological system. Al2O3 and Al2O3/ZrO2 bioceramics are recognized as biomaterials due to their improved mechanical properties, high chemical stability and biocompatibility when compared to other materials. However, due to the bioinert nature of Al2O3 and ZrO2, surface coatings are used to improve the interaction of this biomaterial with the host tissue when implanted, in addition to being able to release ions that stimulate cellular responses at the molecular level, through mechanisms not yet fully understood. In this sense, the main objective of this project is to obtain Al2O3 scaffolds and Al2O3/ZrO2 nanocomposites that may present physicochemical and morphological characteristics that, when implanted in lesions or regions of the bone defect, stimulate osteogenesis and lead to the formation of structures similar to the elements of the extracellular matrix, facilitating the implantation, expansion and integration of the bone tissue. To achieve the main objective, this project was divided into three subprojects. Subproject 1 aims to obtain Al2O3 and Al2O3 scaffolds containing 5vol % of nanometric zirconia inclusions (Al2O3/ZrO2) obtained by gelcasting and 3D printing techniques, coated with calcium phosphates enriched with Sr2+ ions and gentamicin. Subproject 2 aims to cover the Al2O3 and Al2O3/ZrO2 scaffolds with polymeric films carrying bone morphogenetic protein 2 (BMP-2). Subproject 3 aims at the incorporation of mesenchymal stem cells (MSC) in the scaffolds obtained by subprojects 01 and 02, and the functional analysis in vitro and in vivo. With this, it is expected to obtain biocompatible, bioactive and biofunctional Al2O3 and Al2O3/ZrO2 nanocomposites, with potential use in tissue engineering and bone regeneration therapies in veterinary and human medicine, enabling rapid treatment of injured regions. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

LAVAGNINI, ISABELA R.; CAMPOS, JOAO, V; JESUS, LILIAN M.; LOBO, ANDERSON O.; GHOSE, SANJIT K.; RAJ, RISHI; PALLONE, ELIRIA M. J. A.. In-situ synchrotron X-ray diffraction of hydroxyapatite-zirconia composite during conventional and flash sintering. Journal of the European Ceramic Society, v. 44, n. 7, p. 10-pg., 2024-02-24. (19/14677-9, 21/06509-9, 19/03786-1, 22/10604-0)

SANTANA, LEONARDO; NAGATA, ESTER Y.; BARRA, GUILHERME M. O.; NETTO, AURELIO C. S.; PALLONE, ELIRIA M. J.; AMBROSIO, CARLOS E.; SILVEIRA, ZILDA DE CASTRO. Characterization and analysis of printing challenges with high filled alumina filament in low-cost extrusion-based additive manufacturing. Polymer Composites, v. N/A, p. 27-pg., 2025-02-10. (22/10604-0)

SANTOS, SARAH INGRID PINTO; ORTIZ-PENUELA, SANTIAGO JOSE; DE PAULA FILHO, ALESSANDRO; TOMIYAMA, ANA LAURA MIDORI ROSSI; COSER, LILIAN DE OLIVEIRA; DA SILVEIRA, JULIANO COELHO; MARTINS, DANIELE DOS SANTOS; CIENA, ADRIANO POLICAN; DE OLIVEIRA, ALEXANDRE LEITE RODRIGUES; AMBROSIO, CARLOS EDUARDO. Oligodendrocyte precursor cell-derived exosomes combined with cell therapy promote clinical recovery by immunomodulation and gliosis attenuation (vol 18 , 1413843 , 2024). FRONTIERS IN CELLULAR NEUROSCIENCE, v. 18, p. 2-pg., 2025-01-07. (21/09869-6, 22/10604-0)