Biofabrication studies for osteochondral structure

• Grant number: 19/11950-6
• Support Opportunities: Regular Research Grants
• Start date: October 01, 2019
• End date: December 31, 2021
• Field of knowledge: Engineering - Biomedical Engineering - Bioengineering
• Principal Investigator: Jorge Vicente Lopes da Silva
• Grantee: Jorge Vicente Lopes da Silva
• Host Institution: Centro de Pesquisas Renato Archer (CENPRA). Ministério da Ciência, Tecnologia e Inovação (Brasil). Campinas , SP, Brazil
• Associated researchers: Fernando Ely ; Janaina de Andrea Dernowsek ; Juliana Kelmy Macário Barboza Daguano ; Rodrigo Alvarenga Rezende
• Associated scholarship(s): 20/00229-1 - Development and characterization of PEG-Laponite-based biotechnology for 3D printing of chondral structure, BP.TT

Abstract

Biofabrication can be understood as the automated generation of products from the controlled deposition of cells, biomaterials, and biomolecules for the formation of tissues or organs. Biofabrication consists of three main approaches: (1) based on scaffolds, (2) based on tissue spheroids and (3) hybrid. The focus of this project is the hybrid strategy, that is characterized using scaffolds (biodegradable three-dimensional supports for cell growth) that receive the tissue spheroids (three-dimensional structures of pre-established cell clusters) and provide a suitable mechanical and/or biological microenvironment for the development of new tissue. Osteochondral defects, described by a complex stratified nanostructure and contrasting biomechanical properties, are a challenge to be overcome by Biofabrication since cartilage and bone tissues should be mimicked. One of the most efficient ways to obtain scaffolds is Additive Manufacturing, since this processing route allows the material a uniform or more complex geometry and porosity with controlled dimension and morphology of pores with 100% interconnectivity, to appropriately receive the cellular spheroids allowing for the cell's nutrition and withdraw of subproducts of metabolic cell activity. Therefore, the aim of this study is the Biofabrication of the osteochondral structure employing the biphasic biomimetic scaffold design, obtained by an additive manufacturing process, with rigid layers of a highly bioactive glass-ceramic and flexible layers bioprinted with bioink based on polyethylene glycol (PEG). This scaffold should induce in vitro, in its separate layers, the osteogenesis, and chondrogenesis of the tissue spheroids of mesenchymal stem pulp cells (SHED). (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DECARLI, MONIZE CAIADO; AMARAL, ROBSON; DOS SANTOS, DIOGO PERES; TOFANI, LARISSA BUENO; KATAYAMA, ERIC; REZENDE, RODRIGO ALVARENGA; LOPES DA SILVA, JORGE VICENTE; SWIECH, KAMILLA; TORRES SUAZO, CLAUDIO ALBERTO; MOTA, CARLOS; et al. ell spheroids as a versatile research platform: formation mechanisms, high throughput production, characterization and application. BIOFABRICATION, v. 13, n. 3, JUL 2021. (19/11950-6, 15/05102-1)

KARINA FELICIANO SANTO; JOSÉ LUIS DÁVILA; MARCOS AKIRA D'ÁVILA; ANDREA CECÍLIA DORION RODAS; JORGE VICENTE LOPES DA SILVA; JULIANA KELMY MACÁRIO BARBOZA DAGUANO. Estudo da reologia de hidrogéis compósitos de PEG-Laponita-alginato visando impressão 3D baseada em extrusão. MATERIA-RIO DE JANEIRO, v. 27, n. 2, 2022-12-05. (17/23776-5, 19/11950-6)

DECARLI, MONIZE CAIADO; AMARAL, ROBSON; DOS SANTOS, DIOGO PERES; TOFANI, LARISSA BUENO; KATAYAMA, ERIC; REZENDE, RODRIGO ALVARENGA; LOPES DA SILVA, JORGE VICENTE; SWIECH, KAMILLA; TORRES SUAZO, CLAUDIO ALBERTO; MOTA, CARLOS; et al. Cell spheroids as a versatile research platform: formation mechanisms, high throughput production, characterization and applications. BIOFABRICATION, v. 13, n. 3, p. 37-pg., 2021-07-01. (15/05102-1, 19/11950-6)

DAGUANO, JULIANA K. M. B.; GIORA, FABIANA C.; SANTOS, KARINA F.; PEREIRA, ANA B. G. C.; SOUZA, MARINA T.; DAVILA, JOSE L.; RODAS, ANDREA C. D.; SANTOS, CLAUDINEI. Shear-thinning sacrificial ink for fabrication of Biosilicate (R) osteoconductive scaffolds by material extrusion 3D printing. Materials Chemistry and Physics, v. 287, p. 13-pg., 2022-06-02. (19/11950-6, 20/00229-1)

TANIGUCHI NAGAHARA, MARIANA HARUE; CAIADO DECARLI, MONIZE; INFORCATTI NETO, PAULO; LOPES DA SILVA, JORGE VICENTE; MORAES, ANGELA MARIA. Crosslinked alginate-xanthan gum blends as effective hydrogels for 3D bioprinting of biological tissues. Journal of Applied Polymer Science, v. 139, n. 28, p. 15-pg., 2022-05-19. (19/11950-6)