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Development of strategies for Regenerative Dentistry based on three-dimentional printing technologies

Abstract

Tissue Engineering has emerged as an exciting field in Regenerative Medicine to propose alternative methods to regenerate tissues and organs using innovative biofabrication and cell culture methods. Three-dimensional (3D) printing has opened incredible possibilities to provide precise internal and external shape control of biomaterials. In this project, we propose the translation of additive manufacturing technologies to create Tissue Engineering strategies applicable to the Regenerative Dentistry field. Therefore, it will be proposed the application of several 3D printing methods to create biomimetic multifunctional chitosan and gelatin-methacryloyl (GelMA) scaffolds. Indirect and direct printing technologies, using conventional 3D printers and bioprinters, respectively, encompassing different printing technologies, will be used to modulate internal and external scaffold shape applicable for dentin, pulp, and bone Tissue Engineering in a cell-homing strategy. Also, bioprinting will be proposed by using SLA-based bioprinters to allow development of highly precise biomaterials. Personalization proposals of scaffolds using prototyping will also be tested. (AU)

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