Screening of plants with antimicrobial activity against enterobacteria, Pseudomonas spp. and Staphylococcus spp.

Autor(es)

Girondi, Camila Miorelli ; de Oliveira, Analu Barros ; Prado, Jackeline Alves ; Koga-Ito, Cristiane Yumi ; Borges, Aline Chiodi ; Delbem, Alberto Carlos Botazzo ; Pereira, Daniel Freitas Alves ; Salvador, Marcos Jose ; Brighenti, Fernanda Lourencao

• Tipo de documento: Artigo Científico
• Fonte: FUTURE MICROBIOLOGY; v. 12, n. 8, p. 671-681, JUN 2017.
• Citações Web of Science: 3

Resumo

Aim: This study screened plants for antibacterial properties against bacteria of medical importance. Materials \& methods: 60 extracts were obtained from the leaves of ten plants (Jatropha weddelliana, Attalea phalerata, Buchenavia tomentosa, Croton doctoris, Mouriri elliptica, Mascagnia benthamiana, Senna aculeata, Unonopis guatterioides, Allagoptera leucocalyx and Bactris glaucescens) using different extraction methods: A) Ethanol 70 degrees C/72 h; B) Water/5 min/100 degrees C; C) Water/1 h/55 degrees C; D) Water/72 h; E) Hexane/72 h and F) Ethanol 99 degrees C/72 h. Enterobacteria/Pseudomonas and staphylococci reference strains and 201 clinical isolates were used. Primary screening was done using agar well-diffusion assay. MIC/minimum bactericidal concentration and chemical characterization were determined. Results: Extracts 5F and 3A showed the best MIC/minimum bactericidal concentration against clinical isolates and showed the presence of phenols. Conclusion: The present study demonstrated that Mouriri elliptica and Buchenavia tomentosa were the most active plants against the studied bacteria. (AU)

• Processo FAPESP: 12/05234-7 - Triagem de plantas provenientes do Pantanal brasileiro com atividade antimicrobiana contra enterobactérias, Pseudomonas spp. e Staphylococcus spp.
• Beneficiário: Fernanda Lourenção Brighenti
• Modalidade de apoio: Auxílio à Pesquisa - Regular