Bacteriophage use to control Salmonella biofilm on surfaces present in chicken slaughterhouses

Autor(es)

de Ornellas Dutka Garcia, Keila Carolina ; de Oliveira Correa, Isadora Mainieri ; Pereira, Larissa Quinto ; Silva, Tarcisio Macedo ; Ribeiro Mioni, Mateus de Souza ; de Moraes Izidoro, Ana Carolina ; Vellano Bastos, Igor Henrique ; Marietto Goncalves, Guilherme Augusto ; Okamoto, Adriano Sakai ; Andreatti Filho, Raphael Lucio

• Tipo de documento: Artigo Científico
• Fonte: POULTRY SCIENCE; v. 96, n. 9, p. 3392-3398, SEP 2017.
• Citações Web of Science: 7

Resumo

Foodborne diseases represent a major risk to public health worldwide. Pathogenic bacteria can live in the form of biofilm within the food industry, providing a permanent source of contamination. The aim of this study was to evaluate the influence of the types of adhesion surfaces on Salmonella biofilm formation at eight different times, and analyze the action time of a bacteriophage pool on established biofilms. Most of the samples used were classified as weak biofilm producers, with serovars Enteritidis and Heidelberg showing the highest frequency of biofilm formation. Glass and stainless steel surfaces significantly favored biofilm formation at 60 and 36 h of incubation respectively, but the polyvinyl chloride surface did not favor biofilm production, suggesting that the type of material may interfere with production. The bacteriophage pool action period focused on 3 h, but treatment of 9 h on glass surface biofilms was superior to other treatments because it affected the largest number of samples. These results suggests that some surface types and Salmonella serotypes may promote biofilm formation and indicate bacteriophages as an alternative to control biofilms. But further studies are required to prove the effectiveness and safety of bacteriophage therapy as an alternative in the antimicrobial control in the processing plants. (AU)

• Processo FAPESP: 13/07129-9 - Utilização de bacteriófagos ambientais no controle de biofilmes de Salmonella spp. em superfícies (aço inoxidável, polivinil cloreto e vidro)na indústria de processamento e comercialização de carcaças de frango de corte e derivados
• Beneficiário: Keila Carolina de Ornellas Dutka Garcia
• Modalidade de apoio: Bolsas no Brasil - Mestrado