| Texto completo | |
| Autor(es): |
de Oliveira, Ricardo A. G.
;
Materon, Elsa M.
;
Melendez, Matias E.
;
Carvalho, Andre L.
;
Faria, Ronaldo C.
Número total de Autores: 5
|
| Tipo de documento: | Artigo Científico |
| Fonte: | ACS APPLIED MATERIALS & INTERFACES; v. 9, n. 33, p. 27433-27440, AUG 23 2017. |
| Citações Web of Science: | 18 |
| Resumo | |
Breast cancer is the most common cancer in women worldwide. The detection of biomarkers has played a significant role in the early,diagnosis and prognosis of breast cancer. Herein, we describe the construction of a disposable microfluidic immunoarray device (D mu ID) for the rapid and low-cost detection of CAL5-3 (carbohydrate antigen 15-3), a protein biomarker for breast cancer. The D mu ID was constructed using a simple and rapid prototyping technique and was applied to detect CA15-3. in cancer patients, The D mu ID Construction was. based on the use, of a double-sided adhesive card with a microfluidic channel and a screen-printed array with 8 electrodes. Beth? the immunoarray and micro fluidic Channel were designed using an inexpensive home Cutter printer and using low-cost Materials. The immunoarray was modified using the layer-by-layer technique aiming at immobilizing the primary antibody. For the biomarker detection, magnetic particles (MPs) modified with polyclonal antibodies and peroxidase enzymes were used as a strategy for capture, separation, and preconcentration of the biomarker, in addition to :amplification of the electroanalytical signal. The preconcentration and amplification strategies integrated with the nanostructured immunosensors of the D mu ID meaningfully contributed toward the detection of CA15-3 with a limit of detection (LoD) of 6 mu U-4 mL(-1), requiring as low as mu L of serum samples for 8 simultaneous detections. The obtained LoD was 1200 times lower compared to those of other immunosensors previously reported in the literature. The D mu ID was applied for the detection of CA15-3 in real samples of breast cancer patients and was found to present an excellent correlation with the well-established commercial electrochemiluminescence immunoassay.. The association of the D mu ID with nanostructured surfaces and analyte capturing with bioconjugated paramagnetic particles is essentially a promising breakthrough for the low-cost and accurate detection of cancer biomarkers. (AU) | |
| Processo FAPESP: | 15/19890-1 - Desenvolvimento de métodos para diagnóstico de doenças utilizando partículas magnéticas e arranjo de imunossensores em sistema microfluídico descartável |
| Beneficiário: | Ronaldo Censi Faria |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |