Disposable Microfluidic Immunoarray Device for Sensitive Breast Cancer Biomarker Detection

Author(s)

de Oliveira, Ricardo A. G. ; Materon, Elsa M. ; Melendez, Matias E. ; Carvalho, Andre L. ; Faria, Ronaldo C.

• Document type: Journal article
• Source: ACS APPLIED MATERIALS & INTERFACES; v. 9, n. 33, p. 27433-27440, AUG 23 2017.
• Web of Science Citations: 18

Abstract

Breast cancer is the most common cancer in women worldwide. The detection of biomarkers has played a significant role in the early,diagnosis and prognosis of breast cancer. Herein, we describe the construction of a disposable microfluidic immunoarray device (D mu ID) for the rapid and low-cost detection of CAL5-3 (carbohydrate antigen 15-3), a protein biomarker for breast cancer. The D mu ID was constructed using a simple and rapid prototyping technique and was applied to detect CA15-3. in cancer patients, The D mu ID Construction was. based on the use, of a double-sided adhesive card with a microfluidic channel and a screen-printed array with 8 electrodes. Beth? the immunoarray and micro fluidic Channel were designed using an inexpensive home Cutter printer and using low-cost Materials. The immunoarray was modified using the layer-by-layer technique aiming at immobilizing the primary antibody. For the biomarker detection, magnetic particles (MPs) modified with polyclonal antibodies and peroxidase enzymes were used as a strategy for capture, separation, and preconcentration of the biomarker, in addition to :amplification of the electroanalytical signal. The preconcentration and amplification strategies integrated with the nanostructured immunosensors of the D mu ID meaningfully contributed toward the detection of CA15-3 with a limit of detection (LoD) of 6 mu U-4 mL(-1), requiring as low as mu L of serum samples for 8 simultaneous detections. The obtained LoD was 1200 times lower compared to those of other immunosensors previously reported in the literature. The D mu ID was applied for the detection of CA15-3 in real samples of breast cancer patients and was found to present an excellent correlation with the well-established commercial electrochemiluminescence immunoassay.. The association of the D mu ID with nanostructured surfaces and analyte capturing with bioconjugated paramagnetic particles is essentially a promising breakthrough for the low-cost and accurate detection of cancer biomarkers. (AU)

• FAPESP's process: 15/19890-1 - Development of methods for diagnosis of diseases using magnetic particles and an array of imunossensors in a disposable microfluidic system
• Grantee: Ronaldo Censi Faria
• Support Opportunities: Regular Research Grants