Monitoring of prostate cancer biomarkers using a biosensor

• Grant number: 16/15919-8
• Support Opportunities: Regular Research Grants
• Duration: November 01, 2016 - October 31, 2018
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal Investigator: Valber de Albuquerque Pedrosa
• Grantee: Valber de Albuquerque Pedrosa
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated scholarship(s): 18/00959-0 - Development of aptameric sensors for cell monitoring of prostatic cells, BP.TT

Abstract

The development of new devices for monitoring cell metabolism and disease diagnosis expanded the research on biosensors which together with nanotechnology mainly been enable the creation of new elements with high detection sensitivity, specificity, and multiplexing capacity for use in portable devices different areas. Thus, the monitoring of products secreted by the cells via biosensors have great interest in biomedical and health areas, therefore, cells transmit a wide variety of physical and chemical signals that are critical to the clinical diagnosis. Current studies evaluating biocompatibility and cell responses to physical or chemical stimuli are evaluated in microfluidic devices and electrochemical means. Here, we will develop a biosensor using aptamers to study different cell markers released by prostate cancer cells. Thus, the objective of this proposal is to develop a platform for an integrated biosensor to monitor detection of multi-analytes (PSA, fPSA, hK2, mucins and vascular endothelial growth factor, VEGF) in a device manufactured where the cellular microenvironment can be precisely defined, allowing the evaluation of energy metabolism of cell cultures in the context of mimicking the cellular microenvironment and offering new insights into the molecular metabolism events. (AU)

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

KARPIK, AGNIESZKA E.; CRULHAS, BRUNO P.; RODRIGUES, CAROLINA B.; CASTRO, GUSTAVO R.; PEDROSA, VALBER A.. Aptamer-based Biosensor Developed to Monitor MUC1 Released by Prostate Cancer Cells. Electroanalysis, v. 29, n. 10, p. 2246-2253, OCT 2017. (16/15919-8)

BASSO, CAROLINE RODRIGUES; CRULHAS, BRUNO PEREIRA; CASTRO, GUSTAVO ROCHA; PEDROSA, VALBER ALBUQUERQUE. A Study of the Effects of pH and Surfactant Addition on Gold Nanoparticle Aggregation. Journal of Nanoscience and Nanotechnology, v. 20, n. 9, p. 5458-5468, SEP 2020. (16/15919-8)

PARRA, JOAO P. R. L. L.; CRULHAS, BRUNO P.; BASSO, CAROLINE R.; DELELLA, FLAVIA K.; CASTRO, GUSTAVO R.; PEDROSA, VALBER A.. Using an Electrochemical Aptasensor to Early Detect Prostate Specific and Free Prostate Specific Antigens Released by Cancer Cells. Electroanalysis, v. 30, n. 12, p. 2869-2877, DEC 2018. (16/25749-2, 16/15919-8)

CRULHAS, BRUNO P.; KARPIK, AGNIESZKA E.; DELELLA, FLAVIA K.; CASTRO, GUSTAVO R.; PEDROSA, VALBER A.. Electrochemical aptamer-based biosensor developed to monitor PSA and VEGF released by prostate cancer cells. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, v. 409, n. 29, p. 6771-6780, NOV 2017. (16/15919-8)

CRULHAS, BRUNO P.; RECCO, LUCAS C.; DELELLA, FLAVIA K.; PEDROSA, VALBER A.. A Novel Superoxide Anion Biosensor for Monitoring Reactive Species of Oxygen Released by Cancer Cells. Electroanalysis, v. 29, n. 5, p. 1252-1257, MAY 2017. (16/15919-8)

CRULHAS, BRUNO P.; BASSO, CAROLINE R.; PARRA, JOAO P. R. L. L.; CASTRO, GUSTAVO R.; PEDROSA, VALBER A.. Reduced Graphene Oxide Decorated with AuNPs as a New Aptamer-Based Biosensor for the Detection of Androgen Receptor from Prostate Cells. JOURNAL OF SENSORS, 2019. (16/25749-2, 16/15919-8)

BASSO, CAROLINE R.; CRULHAS, BRUNO P.; MAGRO, MASSIMILIANO; VIANELLO, FABIO; PEDROSA, VALBER A.. A new immunoassay of hybrid nanomater conjugated to aptamers for the detection of dengue virus. Talanta, v. 197, p. 482-490, MAY 15 2019. (16/15919-8)