| Grant number: | 16/15919-8 |
| Support type: | Regular Research Grants |
| Duration: | November 01, 2016 - October 31, 2018 |
| Field of knowledge: | Physical Sciences and Mathematics - Chemistry |
| Principal Investigator: | Valber de Albuquerque Pedrosa |
| Grantee: | Valber de Albuquerque Pedrosa |
| Home Institution: | Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil |
| Associated scholarship(s): | 18/00959-0 - Development of aptameric sensors for cell monitoring of prostatic cells, BP.TT |
Abstract
The development of new devices for monitoring cell metabolism and disease diagnosis expanded the research on biosensors which together with nanotechnology mainly been enable the creation of new elements with high detection sensitivity, specificity, and multiplexing capacity for use in portable devices different areas. Thus, the monitoring of products secreted by the cells via biosensors have great interest in biomedical and health areas, therefore, cells transmit a wide variety of physical and chemical signals that are critical to the clinical diagnosis. Current studies evaluating biocompatibility and cell responses to physical or chemical stimuli are evaluated in microfluidic devices and electrochemical means. Here, we will develop a biosensor using aptamers to study different cell markers released by prostate cancer cells. Thus, the objective of this proposal is to develop a platform for an integrated biosensor to monitor detection of multi-analytes (PSA, fPSA, hK2, mucins and vascular endothelial growth factor, VEGF) in a device manufactured where the cellular microenvironment can be precisely defined, allowing the evaluation of energy metabolism of cell cultures in the context of mimicking the cellular microenvironment and offering new insights into the molecular metabolism events. (AU)