Dystrophic phenotype improvement in the diaphragm muscle of mdx mice by diacerhein

Autor(es)

Mancio, Rafael Dias ; Hermes, Tulio de Almeida ; Macedo, Aline Barbosa ; Mizobuti, Daniela Sayuri ; Rupcic, Ian Feller ; Minatel, Elaine

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 12, n. 8 AUG 7 2017.
• Citações Web of Science: 3

Resumo

Chronic inflammation and oxidative stress are striking features of Duchenne muscular dystrophy disease. Diacerhein is an anthraquinone, which exhibits anti-inflammatory and antioxidant properties. Based on their actions, the present study evaluated the effects of diacerhein against myonecrosis, oxidative stress and inflammatory response in the diaphragm muscle of mdx mice and compared these results to current treatment widely used in DMD patients, with a main focus on the impact of prednisone. The results demonstrated that diacerhein treatment prevented muscle damage indicated by a decrease in the IgG uptake by muscle fibers, lower CK levels in serum, reduction of fibers with central nuclei with a concomitant increase in fibers with peripheral nuclei. It also had an effect on the inflammatory process, decreasing the inflammatory area, macrophage staining and TNF-alpha and IL-1 beta content. Regarding oxidative stress, diacerhein treatment was effective in reducing the ROS and lipid peroxidation in the diaphragm muscle from mdx mice. Compared to prednisone treatment, our findings demonstrated that diacerhein treatment improved the dystrophic phenotype in the diaphragm muscle of mdx mice similar to that of glucocorticoid therapy. In this respect, this work suggests that diacerhein has a potential use as an alternative drug in dystrophino-pathy treatment and recommends that its anti-inflammatory and antioxidants properties in the dystrophic muscle should be better understood. (AU)

• Processo FAPESP: 14/01970-6 - Efeito da Diacereína e vitamina E sobre fibras musculares distróficas de camundongos mdx
• Beneficiário: Rafael Dias Mâncio
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 13/17732-4 - Efeito in vivo e in vitro de antiinflamatório e antioxidante em fibras musculares distróficas de camundongos Mdx
• Beneficiário: Elaine Minatel
• Modalidade de apoio: Auxílio à Pesquisa - Regular