Thermosensitive Poly(N-vinylcaprolactam) Injectable Hydrogels for Cartilage Tissue Engineering

Autor(es)

Sala, Renata L. ; Kwon, Mi Y. ; Kim, Minwook ; Gullbrand, Sarah E. ; Henning, Elizabeth A. ; Mauck, Robert L. ; Camargo, Emerson R. ; Burdick, Jason A.

• Tipo de documento: Artigo Científico
• Fonte: TISSUE ENGINEERING PART A; v. 23, n. 17-18, p. 935-945, SEP 2017.
• Citações Web of Science: 16

Resumo

Injectable hydrogels have gained prominence in the field of tissue engineering for minimally invasive delivery of cells for tissue repair and in the filling of irregular defects. However, many injectable hydrogels exhibit long gelation times or are not stable for long periods after injection. To address these concerns, we used thermosensitive poly(N-vinylcaprolactam) (PNVCL) hydrogels due to their cytocompatibility and fast response to temperature stimuli. Changes in the PNVCL molecular weight and concentration enabled the development of hydrogels with tunable mechanical properties and fast gelation times (<60 s when the temperature was raised from room temperature to physiologic temperature). Chondrocytes (CHs) and mesenchymal stem cells were encapsulated in PNVCL hydrogels and exhibited high viability (similar to 90%), asmonitored by Live/Dead staining and Alamar Blue assays. Three-dimensional constructs of CH-laden PNVCL hydrogels supported cartilage-specific extracellular matrix production both in vitro and after subcutaneous injection in nude rats for up to 8 weeks. Moreover, biochemical analyses of constructs demonstrated a time-dependent increase in glycosaminoglycans (GAGs) and collagen, which were significantly augmented in the implants cultured in vivo. Histological analyses also demonstrated regular distribution of synthesized cartilage components, including abundant GAGs and type II collagen. The findings from this study demonstrate thermosensitive PNVCL as a candidate injectable biomaterial to deliver cells for cartilage tissue engineering. (AU)

• Processo FAPESP: 15/07185-1 - Desenvolvimento de nanocompósitos poliméricos aplicados em engenharia tecidual
• Beneficiário: Renata Lang Sala
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 13/25663-2 - Desenvolvimento de Nanocompósitos Poliméricos Aplicados em Engenharia Tecidual
• Beneficiário: Renata Lang Sala
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 13/07296-2 - CDMF - Centro de Desenvolvimento de Materiais Funcionais
• Beneficiário: Elson Longo da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs