Biosurfactant production by Aureobasidium pullulans in stirred tank bioreactor: New approach to understand the influence of important variables in the process

Autor(es)

Brumano, Larissa Pereira ; Fernandes Antunes, Felipe Antonio ; Souto, Sara Galeno ; dos Santos, Julio Cesar ; Venus, Joachim ; Schneider, Roland ; da Silva, Silvio Silverio

• Tipo de documento: Artigo Científico
• Fonte: Bioresource Technology; v. 243, p. 264-272, NOV 2017.
• Citações Web of Science: 8

Resumo

Surfactants are amphiphilic molecules with large industrial applications produced currently by chemical routes mainly derived from oil industry. However, biotechnological process, aimed to develop new sustainable process configurations by using favorable microorganisms, already requires investigations in more details. Thus, we present a novel approach for biosurfactant production using the promising yeast Aureobasidium pullulans LB 83, in stirred tank reactor. A central composite face-centered design was carried out to evaluate the effect of the aeration rate (0.1-1.1 min(-1)) and sucrose concentration (20-80 g. L-1) in the biosurfactant maximum tensoactivity and productivity. Statistical analysis showed that the use of variables at high levels enhanced tensoactivity, showing 8.05 cm in the oil spread test and productivity of 0.0838 cm. h(-1). Also, unprecedented investigation of aeration rate and sucrose concentration relevance in biosurfactant production by A. pullulans in stirred tank reactor was detailed, demonstrating the importance to establish adequate conditions in bioreactors, aimed to scale-up process. (C) 2017 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 14/27055-2 - Intensificação de processos em biorrefinarias de lignocelulósicos integrando etapas de produção de etanol e xilitol a partir de bagaço de cana-de-açúcar em reator de leito fluidizado
• Beneficiário: Felipe Antonio Fernandes Antunes
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 15/06238-4 - Biossurfactantes como moléculas versáteis: um novo conceito de produtos para biorrefinarias e combate às doenças tropicais negligenciadas fase 1: seleção de leveduras, produção fermentativa e caracterização físico-química
• Beneficiário: Silvio Silvério da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular