Teneurin-2 presence in rat and human odontoblasts

Autor(es)

Torres-da-Silvali, K. R. ^[1] ; Tessarin, G. W. L. ^[1] ; Dias, C. A. ^{[2, 3]} ; Guiati, I. Z. ^{[2, 1]} ; Ervolino, E. ^[2] ; Goncalves, A. ^[2] ; Beneti, I. M. ^[4] ; Lovejoy, D. A. ^[5] ; Casatti, C. A. ^{[2, 1]}

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ, Inst Biosci Botucatu, Botucatu, SP - Brazil

^[2] Sao Paulo State Univ, Sch Dent Aracatuba, Basic Sci Dept, Aracatuba, SP - Brazil

^[3] Sao Paulo State Univ, Sch Dent Aracatuba, Restorat Dent Dept, Aracatuba, SP - Brazil

^[4] Sao Paulo State Univ, Sch Dent Aracatuba, Dept Surg & Integrated Clin, Aracatuba, SP - Brazil

^[5] Univ Toronto, Cell & Syst Biol Dept, Toronto, ON - Canada

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 12, n. 9 SEP 19 2017.
• Citações Web of Science: 3

Resumo

Teneurins are transmembrane proteins consisting of four paralogues (Ten-1-4), notably expressed in the central nervous system during development. All teneurins contain a bioactive peptide in their carboxyl terminal named teneurin C-terminal associated peptide (TCAP). The present study analyzed the detailed distribution of teneurin-2-like immunoreactive (Ten-2-LI) cells in developing and mature rat molar teeth, as well as in mature human dental pulps. Ten-2 and TCAP-2 genic expressions were also evaluated in rat and human dental pulps. Finally, Ten-2-LI cells were analyzed during the repair process after dentinpulp complex injury in rat lower molar teeth. For this, histological sections of rat molar teeth and human dental pulps were submitted to immunohistochemical techniques, while total RNA from developing rat teeth and mature human dental pulps were submitted to conventional RT-PCR. Ten-2-LI cells were evident in the initial bell stage of rat molar teeth development, especially in ectomesenchymal cells of the dental papilla. Ten-2-LI odontoblasts showed strong immunoreactivity in rat and human mature teeth. Ten-2 and TCAP-2 genic expressions were confirmed in rat and human dental pulps. Dentin-pulp complex injury resulted in a decrease of Ten-2-LI odontoblasts after traumatic injury. Interestingly, Ten-2-LI cells were also evident in the pulp cell-rich zone in all postoperative days. In conclusion, Ten-2-LI presence in rat and human odontoblasts was demonstrated for the first time and Ten-2/TCAP-2 genic expressions were confirmed in rat and human dental pulps. Furthermore, it was revealed that Ten-2-LI rat odontoblasts can be modulated during the regenerative process. (AU)

• Processo FAPESP: 12/08833-9 - Expressão das teneurinas (TENS) e dos peptídeos associados ao terminal carboxila das teneurinas (TCAPs) no sistema nervoso central de primatas não-humanos (Cebus apella)
• Beneficiário: Kelly Regina Torres da Silva
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 12/03067-6 - Expressão das teneurinas (TEN) e dos peptídeos associados ao terminal carboxila das teneurinas (TCAP) no sistema nervoso central primatas não-humanos (Cebus apella)
• Beneficiário: Cláudio Aparecido Casatti
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/01288-8 - Identificação dos membros da família das teneurinas em órgãos de primatas não-humanos e roedores
• Beneficiário: Isabella Zacarin Guiati
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica