Phototoxicity in a laryngeal cancer cell line enhanced by a targeting amphiphilic chlorin photosensitizer

Autor(es)

Moritz, Milene N. O. ^[1] ; Rossa Junior, Carlos ^[2] ; de Oliveira, Kleber T. ^[3] ; Uliana, Marciana P. ^[3] ; Perussi, Janice R. ^{[4, 1]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Programa Posgrad Interunidades Bioengn EESC FMRP, Sao Carlos, SP - Brazil

^[2] Univ Estadual Paulista Mesquita Filho, Dept Diagnost & Cirurgia, Araraquara, SP - Brazil

^[3] Univ Fed Sao Carlos, Dept Quim, Sao Carlos, SP - Brazil

^[4] Univ Sao Paulo, Inst Quim Sao Carlos, Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Photodiagnosis and Photodynamic Therapy; v. 19, p. 355-362, SEP 2017.
• Citações Web of Science: 0

Resumo

Background: Photodynamic therapy (PDT) has been established in several countries as an alternative therapy for the treatment of various malignancies. This therapy involves the incorporation of a photosensitizer (PS) that is activated by visible light and form reactive oxygen species leading to target cell death by apoptosis or necrosis. Previously, our group has demonstrated that CHL-T (semi-synthesized from chlorophyll a and containing a linked solubilizing group TRISMA (R)) presented a pronounced potential to induce death in HeLa cell line after PDT. In the present study, besides confirm the high cytotoxicity in another cell line, we have further investigated the cell death mechanisms caused by CHL-T as a photosensitizer in laryngeal carcinoma cells. Methods: Cells were exposed to different concentrations of three photosensitizers, namely, hypericin (HY), unmodified chlorin (CHL) and a synthesized amphiphilic chlorin derivative (CHL-T). PSs accumulation and localization were accessed by fluorescence assays. Photosensitization was induced at 6 J cm(-2) using red LEDs (630 +/- 10 nm). Viability was assessed by mitochondrial function (MTT); whereas apoptosis/necrosis was evaluated by fluorescence microscopy and flow cytometry. Expression of pro-apoptotic p53 protein was studied by Western blot. Results and conclusions: All PS showed similar localization profile in the HEp-2 cells. The use of CHL-T increased the percentage of apoptotic cells and also p53 expression in comparison with the use of HY and CHL as photosensitizers. This study shows a significant effect of CHLT associated with red light (630 +/- 10 nm and 18 mW cm(-2)) irradiation on a cancer cell line, indicating the potential of this amphiphilic chlorin in enhancing the therapeutic effectiveness of Photodynamic Therapy (PDT). (AU)

• Processo FAPESP: 15/21110-4 - Explorando Reações Fotocatalisadas em Condições de Fluxo Contínuo pelo uso de Sistemas Porfirinóides: (C-FlowPhotoChem)
• Beneficiário: Kleber Thiago de Oliveira
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/07276-1 - CEPOF - Centro de Pesquisa em Óptica e Fotônica
• Beneficiário: Vanderlei Salvador Bagnato
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs