Influence of toluene and salinity on biosurfactant production by Bacillus sp.: scale up from flasks to a bench-scale bioreactor

Autor(es)

Souza, E. C. ^[1] ; Vessoni-Penna, T. C. ^[1] ; Al Arni, S. ^[2] ; Dominguez, J. M. ^[3] ; Converti, A. ^[4] ; Oliveira, R. P. de S. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Pharmaceut Sci, Biochem & Pharmaceut Technol Dept, Av Prof LineuPrestes 580, Bl 16, BR-05508900 Sao Paulo - Brazil

^[2] Kind Saudi Univ, Dept Chem Engn, POB 800, Riyadh 11421 - Saudi Arabia

^[3] Vigo Univ, Fac Sci, Dept Chem Engn, Campus Ourense, As Lagoas S-N, Orense - Spain

^[4] Genoa Univ, Pole Chem Engn, Dept Civil Chem & Environm Engn, Via Opera Pia 15, I-16145 Genoa - Italy

• Tipo de documento: Artigo Científico
• Fonte: Brazilian Journal of Chemical Engineering; v. 34, n. 2, p. 395-405, APR-JUN 2017.
• Citações Web of Science: 6

Resumo

ABSTRACT To select the best biosurfactant producer, Pseudomonas putida, Bacillus megatherium, Bacillus licheniformis and Bacillus subtilis were cultured in flasks on media with different salinity [low salinity (LS), Bushnell-Haas (BH) and artificial sea water (SW) media] supplemented or not with toluene as a model pollutant. Toluene inhibited the growth of all microorganisms and stimulated the biosurfactant production. B. subtilis exhibited the best performance, being able to lower the surface tension (ST) in the LS medium to 65.5 mN/min in the absence of toluene, and to 46.5 mN/min in the BH medium in the presence of toluene, corresponding to ST reductions of 13.0 and 27.5 mN/m, respectively. Scaling up the process to a bench-scale fermentor, the best results were obtained in the LS medium, where B. subtilis was able to reduce the toluene concentration from 26.0 to 4.3 g/L within 12 h and ST by 17.2 mN/m within 18 h. The results of this study point out that B. subtilis is an interesting biosurfactant producer, which could be used in the bioremediation of toluene-contaminated water. (AU)

• Processo FAPESP: 13/15065-0 - Produção biotecnológica e avaliação das propriedades de biossurfactantes por Lactococcus lactis CECT-4434 a partir de resíduos agroindustriais
• Beneficiário: Ellen Cristina Souza Vera
• Linha de fomento: Bolsas no Brasil - Doutorado