Development of a probiotic delivery system based on gelation of water-in-oil emulsions

Autor(es)

Franco Picone, Carolina Siqueira ^[1] ; Bueno, Andressa Catozzi ^[1] ; Michelon, Mariano ^[1] ; Cunha, Rosiane Lopes ^[1]

Afiliação do(s) autor(es)

^[1] Univ Campinas UNICAMP, Sch Food Engn, BR-13083862 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: LWT-FOOD SCIENCE AND TECHNOLOGY; v. 86, p. 62-68, DEC 2017.
• Citações Web of Science: 5

Resumo

The protection and delivery of probiotic cultures remain a challenge for the food industry. In this work, a new delivery system was developed based on gellan added to the aqueous phase of a water-in-oil emulsion. The concentration of polysaccharide was evaluated to control gel mechanical properties. Uniaxial compression tests were performed to characterize the mechanical properties of gels, and rheological oscillatory tests were conducted to evaluate gelation time. Different emulsion formulations were evaluated with the aim of producing small microgels. The resistance of microgels to digestion steps was evaluated by size measurements and zeta-potential analyses in in vitro tests. The microgels were stable to oral and gastric digestion, but they were destabilized in enteric fluid. Lactobacillus rhamnosus was encapsulated into the gelled and non-gelled emulsions, and its survival was evaluated after in vitro digestion. The probiotic viability was higher than 77% in gelled emulsion and decreased to 66% when the aqueous phase did not form gel. Our results show that emulsion gelation improved microbial resistance to in vitro digestion conditions. (C) 2017 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 12/14003-9 - Estudo dos mecanismos de emulsificação e de-emulsificação na produção microbiana de biodiesel
• Beneficiário: Carolina Siqueira Franco Picone
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado