| Texto completo | |
| Autor(es): |
Costa, Carolina S.
[1]
;
Bravo, Juliana P.
[1]
;
Ribeiro, Cintia L.
[1, 2]
;
Soprano, Adriana S.
[1]
;
Sassaki, Flavio T.
[1, 3]
;
Maia, Ivan G.
[4, 1]
Número total de Autores: 6
|
| Afiliação do(s) autor(es): | [1] UNESP, Inst Biociencias, Dept Genet, BR-18618689 Botucatu, SP - Brazil
[2] Monsanto Co, 700 Chesterfield Pkwy W, Chesterfield, MO 63017 - USA
[3] Univ Fed Uberlandia, Inst Genet & Bioquim, Campus Monte Carmelo, Rodovia LMG 746, Monte Carmelo, MG - Brazil
[4] UNESP, Inst Biociencias, Dept Genet, BR-18618970 Botucatu, SP - Brazil
Número total de Afiliações: 4
|
| Tipo de documento: | Artigo Científico |
| Fonte: | PLANT CELL TISSUE AND ORGAN CULTURE; v. 131, n. 2, p. 213-222, NOV 2017. |
| Citações Web of Science: | 2 |
| Resumo | |
The construction of expression cassettes harboring tissue-specific promoters is a viable alternative to limit transgene expression to specific organs and cell types. In this study, we have functionally characterized the promoter of a Eucalyptus grandis gene encoding a putative high-affinity HAK5-like potassium (K+) transporter (designated EgHAK5) showing root-specific expression. The ability of the EgHAK 5'-flanking region (similar to 1.3 kb) to drive root-specific expression of a reporter gene (beta-glucuronidase; GUS) was examined using transgenic tobacco plants. Histochemical analysis revealed enhanced GUS staining in the vasculature of leaves, hypocotyls and roots, which was also confirmed in histological cross-sections. Moreover, the relative expression of GUS in the roots of the generated transgenic lines was increased in response to K+ starvation. Overall, our results indicate that, in a heterologous system, the EgHAK5 promoter shows expression in vascular tissues, mainly within the phloem, and is up-regulated upon potassium deprivation. (AU) | |
| Processo FAPESP: | 03/00724-7 - Isolamento e caracterização de promotores tecido-específicos a partir das informações do SUCEST e FOREST |
| Beneficiário: | Ivan de Godoy Maia |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |