Comparative cytogenetics in three Sciaenid species (Teleostei, Perciformes): evidence of interspecific chromosomal diversification

Autor(es)

Xu, Dongdong ^[1] ; Molina, Wagner Franco ^[2] ; Yano, Cassia Fernanda ^[3] ; Zhang, Yurong ^[1] ; de Oliveira, Ezequiel Aguiar ^{[3, 4]} ; Lou, Bao ^[1] ; Cioffi, Marcelo de Bello ^[3]

Afiliação do(s) autor(es)

^[1] Marine Fishery Inst Zhejiang Prov, Key Lab Mariculture & Enhancement Zhejiang Prov, Zhoushan 316100, Zhejiang - Peoples R China

^[2] Univ Fed Rio Grande do Norte, Ctr Biociencias, Dept Biol Celular & Genet, Campus Univ, Lagoa Nova 3000, BR-59078970 Natal, RN - Brazil

^[3] Univ Fed Sao Carlos, Dept Genet & Evolucao, Rodovia Washington Luis, Km 235, BR-13565905 Sao Carlos, SP - Brazil

^[4] Secretaria Estado Educ Mato Grosso SEDUC MT, Cuiaba, MT - Brazil

• Tipo de documento: Artigo Científico
• Fonte: MOLECULAR CYTOGENETICS; v. 10, OCT 23 2017.
• Citações Web of Science: 0

Resumo

Background: Species belonging to the Sciaenidae family present a karyotype composed by 48 acrocentric chromosomes and are thus considered a striking example of chromosomal conservation. In this family, three species are extensively studied including Larimichthys crocea, Larimichthys polyactis and Nibea albiflora due to their importance in fishery and aquaculture in East Asia. Despite abundant data of population genetics available for some of them, cytogenetic information on these species is still scarce and obtained by conventional cytogenetic protocols. Therefore, a more detailed cytogenomic investigation was performed in these species to analyze their karyotype differentiation using conventional staining techniques and fluorescence in situ hybridization to map several repetitive DNAs. Results: The three species showed a slight karyotype differentiation with 4sm + 2st + 42a in L. polyactis, 20st + 28a in L. crocea and 48a in N. albiflora. Additionally, the mapping of repetitive sequences further revealed a number of interspecific differences among them. Particularly, 18S and 5S rDNA sites showed syntenic arrangements in N. albiflora and non-syntenic arrangements in both Larimichthys species. The microsatellites (CA) 15 and (GA) 15 showed conspicuous terminal clusters in some chromosomes of all species. On the other hand, (CGG) 10 repeats, Rex6 elements and U2 snRNA displayed a scattered distribution on the chromosomes. Conclusions: Although the three Sciaenid species examined displayed a general pattern of karyotypic conservatism, we explored chromosomal diversification among them. The diversificated karyotypic macrostructure is followed by intergeneric evolutionary diversification of the repetitive sequences. The data indicate some degree of intergeneric evolutionary diversification at chromosomal level, and suggest the evolutionary dynamics among Sciaenid species, higher than previously thought. The present cytogenetic data provide new insight into the chromosomal diversification in Sciaenidae, and contribute to inferring the chromosomal rearrangements and trends of karyotype evolution in this fish group. (AU)

• Processo FAPESP: 16/22196-2 - Uma abordagem intercontinental para a investigação da evolução cromossômica na ordem Osteoglossiformes (Teleostei: Osteoglossomorpha). Parte II
• Beneficiário: Marcelo de Bello Cioffi
• Modalidade de apoio: Auxílio à Pesquisa - Regular