Polyethylene imine/graphene oxide layer-by-layer surface functionalization for significantly improved limit of detection and binding kinetics of immunoassays on acrylate surfaces

Autor(es)

Miyazaki, Celina M. ^{[1, 2]} ; Mishra, Rohit ^[1] ; Kinahan, David J. ^[1] ; Ferreira, Marystela ^[2] ; Ducree, Jens ^[1]

Afiliação do(s) autor(es)

^[1] Dublin City Univ, Sch Phys Sci, Natl Ctr Sensor Res, Dublin 9 - Ireland

^[2] Univ Fed Sao Carlos, CCTS, Rod Joao Leme dos Santos, Km 110, BR-18052780 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: COLLOIDS AND SURFACES B-BIOINTERFACES; v. 158, p. 167-174, OCT 1 2017.
• Citações Web of Science: 6

Resumo

Antibody immobilization on polymeric substrates is a key manufacturing step for microfluidic devices that implement sample-to-answer automation of immunoassays. In this work, a simple and versatile method to bio-functionalize poly(methylmethacrylate) (PMMA), a common material of such ``Lab-on-a-Chip{''} systems, is proposed; using the Layer-by-Layer (LbL) technique, we assemble nanostructured thin films of poly(ethylene imine) (PEI) and graphene oxide (GO). The wettability of PMMA surfaces was significantly augmented by the surface treatment with (PEI/GO)(5) film, with an 81% reduction of the contact angle, while the surface roughness increased by 600%, thus clearly enhancing wettability and antibody binding capacity. When applied to enzyme-linked immunosorbent assays (ELISAs), the limit of detection of PMMA surface was notably improved from 340 pg mL(-1) on commercial grade polystyrene (PS) and 230 pg mL(-1) on plain PMMA surfaces to 130 pg mL(-1) on (PEI/GO)(5) treated PMMA. Furthermore, the accelerated antibody adsorption kinetics on the LbL films of GO allowed to substantially shorten incubation times, e.g. for anti-rat IgG adsorption from 2 h down to 15 min on conventional and treated surfaces, respectively. (C) 2017 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 15/16311-0 - Detecção altamente paralelizada de bioamostras por SPR em uma plataforma microfluídica integrada para análises em larga escala
• Beneficiário: Celina Massumi Miyazaki
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado