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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Polyethylene imine/graphene oxide layer-by-layer surface functionalization for significantly improved limit of detection and binding kinetics of immunoassays on acrylate surfaces

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Author(s):
Miyazaki, Celina M. [1, 2] ; Mishra, Rohit [1] ; Kinahan, David J. [1] ; Ferreira, Marystela [2] ; Ducree, Jens [1]
Total Authors: 5
Affiliation:
[1] Dublin City Univ, Sch Phys Sci, Natl Ctr Sensor Res, Dublin 9 - Ireland
[2] Univ Fed Sao Carlos, CCTS, Rod Joao Leme dos Santos, Km 110, BR-18052780 Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: COLLOIDS AND SURFACES B-BIOINTERFACES; v. 158, p. 167-174, OCT 1 2017.
Web of Science Citations: 6
Abstract

Antibody immobilization on polymeric substrates is a key manufacturing step for microfluidic devices that implement sample-to-answer automation of immunoassays. In this work, a simple and versatile method to bio-functionalize poly(methylmethacrylate) (PMMA), a common material of such ``Lab-on-a-Chip{''} systems, is proposed; using the Layer-by-Layer (LbL) technique, we assemble nanostructured thin films of poly(ethylene imine) (PEI) and graphene oxide (GO). The wettability of PMMA surfaces was significantly augmented by the surface treatment with (PEI/GO)(5) film, with an 81% reduction of the contact angle, while the surface roughness increased by 600%, thus clearly enhancing wettability and antibody binding capacity. When applied to enzyme-linked immunosorbent assays (ELISAs), the limit of detection of PMMA surface was notably improved from 340 pg mL(-1) on commercial grade polystyrene (PS) and 230 pg mL(-1) on plain PMMA surfaces to 130 pg mL(-1) on (PEI/GO)(5) treated PMMA. Furthermore, the accelerated antibody adsorption kinetics on the LbL films of GO allowed to substantially shorten incubation times, e.g. for anti-rat IgG adsorption from 2 h down to 15 min on conventional and treated surfaces, respectively. (C) 2017 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 15/16311-0 - Highly parallelised SPR detection of biosamples on a large-scale-integrated microfluidic platform
Grantee:Celina Massumi Miyazaki
Support Opportunities: Scholarships abroad - Research Internship - Post-doctor